| Objective:Preterm delivery is one of the most common complications of pregnancy in obstetrical diseases,and there are many factors causing preterm delivery,among which inflammation leads to preterm delivery most obviously.Previous studies have confirmed the blood and maternal-fetal interface of preterm delivery patients.In recent years,Th17/Treg inflammatory axis has become a hot spot,especially in the study of unexplained recurrent abortion diseases,especially in the study of recurrent abortion of unknown causes,especially in the study of recurrent abortion of unknown causes,especially in the study of recurrent abortion of unknown causes,especially in the study of recurrent abortion of unknown causes,especially in the study of recurrent abortion.In addition,it has been shown to play an important role in preterm delivery and other pregnancy diseases.Vitamin D is a fat-soluble trace element that plays a role in bone development,bronchial asthma,lung cancer,obesity and type 2 diabetes.Studies have shown that vitamin D plays an anti-inflammatory role in hormones.The related literature also confirmed the antagonism to the inflammatory factor transforming growth factor（TGF-β1）and tumor necrosis factorα（TNF-α）,and the Th17 cell、Treg cell can also produce these two kinds of inflammatory factors.But the role of preterm delivery has not been further studied.Vitamin D binding protein（VDBP）is a carrier of free vitamin D and can also participate in immune response and viral infection.In this study,SD rats were used to study the relationship between vitamin D and vitamin D binding protein and TGF-β1,which was induced by lipopolysaccharide and induced preterm delivery by culturing animals to mate and conceive,and then drug intervention to establish preterm delivery model of rats with vitamin D and vitamin D binding protein.The relationship between TNF-αinflammatory factors provides a new idea for the treatment of preterm labor.Methods:Clean grade SD rats,9 healthy female rats,weighing about350g/,12 weeks old,5 male rats,15 weeks old,about 500g/,healthy and fertile rats.The male and female rats were free to eat the pellet feed provided by our hospital,keeping the room temperature at 19-26 degrees Celsius and the humidity at 54-66%.The female and male rats closed their cages at night at 2:1and took vaginal smears on the next morning.Vaginal smears showed a large number of sperm on the first day of pregnancy under a 40-fold optical microscope,and if visible to the naked eye,the vaginal suppository was also successful.All pregnant female rats were randomly divided into three groups:normal group（blank group）,model group（LPS group）,experimental group（vitamin D LPS group）.On the 8th day of pregnancy,the experimental group was given vitamin D 600 units per day[1]and fed for 7 days,and the pregnant female rats were divided into four groups:normal group（blank group）,model group（VD group）and experimental group（vitamin D-LPS group）.On the 15th day of gestation,the model group and the experimental group were given intraperitoneal injection of 400ug/kg[2]dose of LPS.The changes of placental inflammation in rats were observed by HE staining microscope.The changes of TGF-β1,TNF-αand vitamin D binding protein in pregnant rats were detected by ELISA method.Results:Both the model group and the experimental group had vaginal bleeding and preterm delivery.The degree of placental HE staining inflammation in the model group was significantly heavier than that in the normal pregnancy group,and the degree of placental inflammation in the experimental group was between the normal group and the model group.The results of ELISA showed that the content of TGF-β1 in serum of normal group was higher than that of model group（P<0 05）.In amniotic fluid,the content of TNF-αin normal group was lower than that in model group（P<0 05）,and the content of TNF-αin experimental group was lower than that in model group（P<0 05）.The vitamin D binding protein in the model group was higher than that in the normal group（P<0.05）.The vitamin D binding protein in the experimental group was lower than that in the model group（P<0.05）.Conclusion:1.Compared with normal pregnant rats,the inflammatory factors of TGF-beta and TNF-alpha in infectious preterm labor rats were relatively reduced and increased.2.The addition of vitamin D reduced TNF-alpha inflammatory cytokines in rats with preterm labor,suggesting that vitamin D may have a protective effect on infectious preterm labor.3.The level of VDBP in pregnant rats of infectious preterm labor model was significantly increased,and the high concentration of vitamin D binding protein was positively correlated with preterm labor.4.Vitamin D supplementation may be combined with more VDBP to reduce its level,thereby inhibiting its chemotaxis to neutrophils. |
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