Study Of Yiqijianpi Traditional Chinese Medicine Compound On Gastric Motility Regulation Mechanism Of The Rats With Diabetic Gastroparesis

Objective To observe the effect of Yiqijianpi traditional Chinese medicine compound on gastric motility,gastrointestinal hormone and SCF,c-Kit protein of diabetic gastroparesis(DGP)rats and explore the possible regulatory mechanism of gastric motility improvement.To provide experimental basis for clinical treatment of DGP.Methods The DGP rat model was established by tail vein injection STZ combined with high fat,high sugar and irregular feeding methods.After 4 weeks,they were randomly divided into model group(normal saline 10 ml/kg),mosapride group(1.575 mg/kg),high dose and low dose of TCM group(5g/kg,1.25g/kg).In addition,normal rats were set as control group(normal saline 10 ml/kg).Control group and model group were given normal saline,while mosapride group,high dose and low dose of TCM group were given corresponding medicine according to the above concentrations.Each group was intragastrically administered once a day for 4 weeks.The general conditions were recorded,and changes in weight,blood glucose,gastric emptying rate and intestinal propulsion rate were monitored.HE staining was used to observe pathological changes of gastric antrum.ELISA was used to detect serum gastrin(GAS),plasma motilin(MTL)and substance P(SP).Immunohistochemistry was used to detect the distribution and expression of tyrosine kinase(c-Kit)in gastric antrum,and the expression of stem cell factor(SCF)and tyrosine kinase(c-Kit)was detected by Western blotting.Results Compared with control group,model group rats general situation became worse,the blood sugar levels increased,and the weight,gastric emptying rate and intestinal propulsion rate decreased.Serum GAS,plasma MTL,SP levels decreased.The expression of SCF and c-Kit protein in gastric antrum decreased(P<0.01).HE staining showed the glands of the gastric antrum were sparse and arranged disorderly,and the number of blood vessels was reduced.Compared with model group,the general situation of high dose of TCM group and mosapride group improved,weight,gastric emptying rate,intestinal propulsion rate increased,serum GAS,plasma MTL,SP content increased.The expression of SCF and c-Kit protein in gastric antrum increased(P<0.05).The blood glucose of the high dose of TCM group decreased(P<0.01).HE staining showed a significant improvement in the mucosal glands and blood vessels of the gastric antrum.Conclusions Yiqijianpi TCM compound can improve the gastric motility of DGP rats and has a dose-effect relationship.The mechanism may be related to promoting the secretion of GAS,MTL,SP and other gastrointestinal hormones and regulating the expression of SCF and c-Kit proteins in gastric antrum.