Expression Of IL-33 In Glioma And Its Correlation With Peritumoral Edema Of Glioma

Background:Gliomas are currently the most common malignant tumors in the central nervous system,accounting for about 50%-60%of all intracranial tumors.With strong invasiveness,high recurrence rate,and poor prognosis,the current surgery,radiotherapy,chemotherapy and other means can not achieve better results.Therefore,more and more researchers are exploring new ideas for the diagnosis and treatment of glioma in the molecular biological characteristics of glioma.IL-33 is a newly discovered interleukin family cytokine and is a novel inflammatory alarm.In many tumors such as liver cancer,stomach cancer,breast cancer,etc.,it is found that IL-33 can secrete a large number of signal pathways such as MAPK/Erk,MAPK/p38 and NF-κB by binding to receptor ST2.Cytokines create a tumor microenvironment that is conducive to tumor growth.At the same time,IL-33,as an important autoimmune factor,can reduce the killing ability of NK cells and CD8+cells in the early and middle stages of tumors,and help tumors complete immune escape.Whether IL-33 has a similar effect in glioma has not been clearly reported.Purpose:ResearchThe expression of IL-33 in gliomas and its correlation with peritumoral edema of glioma.1.Examine the expression of IL-33 in all human tumors in the Oncomine database and compare the glioma datasets in a box shape.IL-33 related reports were searched and screened in PUBMED,coexpression analysis was performed using Coexpedia,and GO and KEGG enrichment analysis was performed using DAVID.2.Study subjects:53 cases of glioma treated by neurosurgery admitted to the Affiliated Hospital of North Sichuan Medical College from March 2017 to November 2018 as the research object,and 9 cases of pathological decompression of craniocerebral injury during the same period As a control group for non-tumor tissues.3.Inclusion criteria:glioma:1)The patient was diagnosed by glioma diagnosis by 2 experienced neuropathologists or pathologists with above-mentioned senior titles.2)No other history of intracranial tumors,inflammation,or autoimmune disease.Normal brain tissue:1)Patients with acute brain trauma requiring internal decompression.2)No history of intracranial tumors,inflammation,and autoimmune disease.4.Specimen collection:Surgical specimens for patients with gliomas and craniocerebral injury requiring internal decompression were collected before surgery,and stored in a refrigerator at-80°C after liquid nitrogen transport.Mainly used for mRNA and immunohistochemistry of IL-33.5.Glioma peritumoral edema determination:This study mainly determined the patient’s tumor and edema boundary by mri scan+enhancement of the patient,and used the elliptic volume algorithm to obtain the volume of tumor and edema.Finally,the degree of edema is expressed by the ei index ei=(v_Edema+v_tumor)/v_tumor.The mRNA and protein expression of IL-33 was detected by RT-PCR.The expression of IL-33 mRNA in glioma and control group was detected by immunohistochemical staining.result:1.Excavating the expression of IL-33 in glioma in Oncomine and found that IL-33 was significantly higher than the normal control group.The expressions of IL-33 mRNA and IL-33 protein in 53 glioma tissues and 9normal brain tissues of our hospital were detected by RT-PCR and immunohistochemistry.Compared with normal brain tissue,IL-33 expression was significantly increased in glioma tissues at both protein and mRNA levels（P<0.05）.And the expression level of IL-33 increased with the increase of glioma grade.2.According to the mRNA expression of IL-33,it was divided into IL-33 high expression group（>3.05）and low expression group（≤3.05）.The expression of IL-33 had no significant relationship with the age,sex,tumor size and tumor location of the patients（all P>0.05）.3.By comparing the expression of IL-33 mRNA and the EI value of peritumoral edema,it was found that the peritumoral edema index of IL-33high expression group was significantly higher than that of low expression group,and the expression of IL-33 was related to glioma.The degree of peritumoral edema was positively linear（r=0.73;P=0.002）.Therefore,the expression level of IL-33 is closely related to glioma peritumoral edema.Gene enrichment analysis of IL-33 revealed that IL-33 may participate in the formation and progression of peritumoral edema through MAPK and NF-NF-κB.Conclusion:1.IL-33 is highly expressed in glioma tissues and is closely related to the grade of glioma.The higher the grade of glioma,the higher the expression of il-33 may be one of the risk factors for the incidence and malignancy of glioma.2.The expression of IL-33 is positively correlated with the degree of edema around glioma.IL-33 may participate in the formation of peritumoral edema through MAPK and NF-κB pathway.