| Objective: To investigate the expression of c-Jun N-terminal kinase 2(JNK2),discoidin domain receptor 1(DDR1),matrix metalloproteinase 9(MMP9)in meningiomas and to analyze the relationship between peritumoral brain edema(PTBE)and invasiveness of meningiomas and the above-mentioned proteins and clinicopathological features.Methods:(1)92 cases of meningiomas that were diagnosed by pathology and met the inclusion and exclusion criteria were collected from October 2016 to December 2018.(2)Collecting 8 cases of arachnoid tissues from patients with traumatic cerebral hematoma in our hospital from June 2018 to December 2018.(3)Immunohistochemistry was used to detect the expression of JNK2,DDR1 and MMP9 in meningioma and traumatic arachnoid tissues,and to analyze the relationship between PTBE and invasiveness of meningiomas and the above-mentioned proteins and clinicopathological features.Result: 1.The positive expression of JNK2 in meningiomas tissues was located in the cytoplasm,nucleus.In 92 cases of meningiomas,the integral optical density(IOD)of JNK2 was 2865.3(991.4-5955.2).In 8 cases of traumatic arachnoid tissues,the IOD of JNK2 was 700.7(592.0-845.3),the difference was statistically significant(P=0.013<0.05);the positive expression of DDR1 in meningiomas was located in the cytoplasm and cell membrane.In 92 cases of meningiomas,the IOD of DDR1 was 1207.8(252.8-3098.8).In 8 cases of traumatic arachnoid tissues,the IOD of DDR1 was 75.3(52.2-177.8),the difference was statistically significant(P=0.001<0.05);the positive expression of MMP9 in meningioma was located in the cytoplasm,cell membrane.In 92 cases of meningioma tissues,the IOD of MMP9 was 57.3(7.0-486.4).In 8 cases of traumatic arachnoid tissues,the IOD of MMP9 was 6.6(0.4-15.4),the difference was statistically significant(P=0.009<0.05).2.The incidence of PTBE in male and tumor diameter ≥4cm was higher than that in female,tumor diameter <4cm,the difference was statistically significant(P<0.05);the incidence of PTBE in age,location,tumor grade,tumor invasiveness,the difference was no statistically significant(P>0.05).3.In 92 cases of meningiomas,there were 50 cases of edema and no edema in 42 cases.The IOD of JNK2,DDR1 and MMP9 in edema group were 4015.1(1124.8-8473.0),2244.0(723.8-7320.4)and 286.1(32.2-5744.2),respectively.The IOD in the non-edema group was 2054.5(853.5-4211.2),535.5(118.5-1589.1),and 14.4(2.0-241.9),respectively.The statistical analysis showed that the P values were 0.067,<0.001,<0.001,and the expression difference of JNK2 was not Statistical significance(P>0.05),the expression difference of DDR1 and MMP9 was statistically significant(P<0.05).4.The incidence of high-grade meningioma invasion was higher than that of low-grade meningioma.The difference was statistically significant(P<0.05).The incidence of meningioma invasion was no statistically significant differences in gender,age,diameter,location,and PTBE(P>0.05).5.In 92 cases of meningiomas,66 cases of invasion occurred,26 cases of no invasion.The IOD of JNK2,DDR1 and MMP9 in the invasive group were 3088.4(1329.8-8473.0),1979.6(459.1-4877.7),164.2(9.0-654.2),respectively.The IOD in the non-invasive group were 1603.4(270.0-4738.5),1069.4(186.7-2737.4),and 17.2(6.2-289.9),respectively.The statistical analysis showed that the P values were 0.036,0.499,and 0.349,respectively.The difference in expression of JNK2 was statistically significant(P<0.05),the difference in expression of DDR1 and MMP9 was not statistically significant(P>0.05).6.There was a positive correlation between JNK2 and DDR1(r=0.518,p<0.01);There was a positive correlation between JNK2 and MMP9(r=0.401,p<0.01);There was a positive correlation between DDR1 and MMP9(r=0.419,p<0.01).Conclusion: 1.JNK2,DDR1 and MMP9 are higher in meningioma than in traumatic arachnoid tissues,suggesting that JNK2,DDR1 and MMP9 are involved in meningioma.2.Gender,tumor size involved in the formation of PTBE,tumor level involved in the invasion and growth of meningioma.3.The expression of JNK2 in the meningioma invasion group was higher than that in the non-invasive group,suggesting that it participates in the invasion and growth of meningioma.4.DDR1,MMP9 in the meningioma edema group was higher than the non-edema group,and they were positively correlated.DDR1 may be involved in the formation of PTBE by mediating MMP 9.5.The expression intensity between JNK2 and DDR1,between JNK2 and MMP9,and between DDR1 and MMP9 were positively correlated,suggesting that combined detection has clinical significance for the diagnosis,treatment and prognosis of meningiomas. |
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