| Objective To observe and test the local and overall situation of rabbits and the function of related organs and tissues by implanting porous magnesium alloy filling material into rabbits,,the safety of the material in rabbits was analyzed.Meanwhile,the bone-promoting ability of the material was analyzed by imaging observation to evaluate the biocompatibility of the material in vivo.Method New Zealand rabbits were implanted with porous magnesium alloy material for left radius bone defect as experimental group,and non-implanted materials were set as control group to compare the following data:1.Observation with naked eyes: observation of wound healing and overall condition;2.Blood sample detection: blood routine,liver and kidney function,inflammatory index and electrolyte,and analysis of the changes of the above-mentioned indicators after implantation;3.Pathological observation: heart,liver and spleen.HE staining was applied to the vital organs of lung,kidney and brain.At the same time,histological observation and analysis were carried out on the organs.4.Imaging examination: X-ray photography was used to observe the formation of bubbles around the material and the growth and degradation of new bone.Result 1.Naked eye observation: the wound healed well,there was no obvious infection and inflammation,and the overall condition of the rabbit was good,no death occurred;2.Serology: The results showed that the blood routine,electrolyte,liver function,renal function and inflammation indexes of the experimental group and the control group were not significantly compared at the same time;3.Pathology: The results showed that there was no significant statistical difference between the experimental group and the control group at the same time stage in the center,liver,spleen,lung,kidney and brain;4.Imaging: The results showed that there was a small amount of gas in the bone defect of the experimental group at 4 weeks after operation,no significant gas at 8 weeks and 12 weeks after operation,and no gas in the control group at all time points.At the same time,the growth of new bone defect in the experimental group was significantly better than that in the blank control group at different time stages.Conclusion1.Porous magnesium alloy with openings is a new type of medical magnesium alloy material with good biocompatibility.2.Porous magnesium alloys with open holes can effectively serve as bone defect support materials and have certain osteogenic effects. |
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