| Purposes : The injury of the bone-tendon junction is more common in daily life and physical training,and it is difficult to heal.The microscopic structure and mechanism of the bone-tendon junction is one of the current research hotspots.However,the existing research on the injury of the bone micro-structure of the bone-tendon junction is more common in the discussion of histomorphology.However,the histological method has a large measurement error and can only reflect a single planar structure,which cannot show the deficiency of the three-dimensional(3D)structure.The Micro-CT technology has the advantages of high resolution,high precision,non-invasiveness and good repeatability.In recent years,it has been widely used in the study of related structural functions of bone tissue,internal organs and vascular organs,etc.At present,the research depth on the 3D structure measurement method of the bone-tendon junction by Micro-CT technology is not inadequate;therefore,this study will establish a method for measuring the three-dimensional structure of the bone-tendon joint by using the operation of Micro-CT technology.Also,the accurate,the 3D measurement methods will provide different perspectives and feasible reference for such research.Methods: The study subjects were 20 adult female New Zealand white rabbits(18 weeks,weight: 2400±200g).They were randomly divided into 2 groups: control group(CON,n=10)and injury group(INJ,n=10).The left hind limb of the INJ and CON groups animal was subjected;taking the materials together with the CON.A Micro-CT scan is performed for the sampling material,and then the reconstructed sample image is reconstructed.According to the previous research and experimental conclusions,the Achilles tendon is up to 200 pieces to the bottom of the bottom 600,that is,a total of 400 pieces are reconstructed using the Nrecon software,and then thereconstructed images is calculated and analyzed by the Ctan software,with ROI size selected as 2×2 mm,and the gray scale is selected,too.The calculation is performed and analyzed with a threshold range of 35-75.The obtained three-dimensional structural method test results are compared with the corresponding H&E staining results to further determine the feasibility.Results: 1.The 3D morphological features of the fibrocartilage band are clear;The change of damage corresponds to the morphological changes before and after the injury of the joint.The results of the means comparations are all significantly different(p<0.01),that is the thickness mean of the cartilage band in the INJ group is significantly thicker than the CON groups’;3.The results of the 3D image method established in this experiment showed that the Tb.N index of the trabecular bone between the CON and the INJis extremely significant(p<0.01).Other indicators: total volume TV,patellar volume BV,patellar surface area BS and trabecular thickness Tb.Th showed no significant difference(p>0.05).Conclusions: 1.The 3D morphological features of the fibrous cartilage zone generated by the three-dimensional structure measurement method can be well observed.The morphological characteristics of the CON and INJ groups are consistent with the changes at the situation when before and after the injury;the 3D structure measurement method are consistent,suggesting that the 3D structure measurement method established in this study is effective and feasible.2.The observation and description of the 3D morphological features of the fibrocartilage band in the bone-tendon junction of this study can be obtained and clearly observed.The changes of the microstructural index of these two methods are consistent,suggesting the selected The ROI and grayscale thresholds are reasonable.Also,the suggestions for the research is: increasing the sample size of the experiment and adopting other test measurement techniques(PET,SPECT,etc.),which maybe alsocan increase the precision of the study. |
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