Effects Of Passive Stretch Movement Combined With Electro Acupuncture On Skeletal Muscle In Mice With Muscle Atrophy Of Disuse

Purpose: In this study,we used passive stretch motor and electroacupuncture to intervene in a mouse model of skeletal muscle disuse atrophy.The histomorphological observation and correlation of troponin blot expression were used to evaluate the efficacy of passive stretch exercise and electroacupuncture,and to explore related rehabilitation mechanisms.It provides a reference for the treatment of muscle atrophy caused by long-term braking during injury rehabilitation.Methods: Fifty C57BL/6 mice were randomly divided into blank control group,model control group,passive stretch exercise group,electroacupuncture intervention group and combined intervention group using a simple random number table,with 10 rats in each group.The blank control group did not undergo muscle atrophy modeling,and the other four groups used plaster to the posterior limb to fix the skeletal muscle disuse muscle atrophy model.After successful modeling,the electroacupuncture intervention group and the passive stretch movement group,combined intervention group were respectively electro-acupuncture and passive stretch movement,passive stretch movement combined electroacupuncture intervention treatment,once a day,10 minutes at a time,intervention for 4 weeks.The mouse gastrocnemius and soleus muscles were taken separately.The cross-sectional area of muscle fibers and the ratio of fast and slow muscle fibers were analyzed by ATPase.The slow skeletal muscle troponin(TNNI1)and fast skeletal muscle troponin(TNNI2)expression were detected by Western Blot.The experimental data were analyzed by SPSS 17.0 statistical software for independent sample T test.The results were significant at P<0.05,and the results were very significant at P<0.01.Results:(1)Recovery of muscle fiber cross-sectional area,from the results of the soleus muscle,compared with the model control group,only the combined intervention group had a significant increase in fast and slow muscle fibers(P<0.05).From the results of the gastrocnemius muscle,the passive stretch exercise group was not significantly different from the model control group(P>0.05).The cross-sectional area of the gastrocnemius muscle in the electroacupuncture intervention group was significantly increased(P<0.05),and the combined intervention group had a very significant increase(P<0.01).(2)For changes in the ratio of muscle fibers,in the results of the soleus muscle,both the electroacupuncture intervention group and the combined intervention group had a decrease in the percentage of fast muscle fibers and an increase in the percentage of slow muscle fibers;in the passive stretch exercise group,the percentage of slow muscle fibers decreased,and the percentage of fast muscle fibers increased.In the results of the gastrocnemius muscle,the ratio of fast and slow muscles in the combined intervention group was similar to that in the blank control group,followed by the electroacupuncture intervention group and finally the passive stretch exercise group.(3)For changes in the relative expression of proteins,compared with the model control group,the expression of TNNI1 and TNNI2 protein in the three intervention groups had no significant effect(P>0.05);the expression of TNNI1 protein and TNNI2 protein in the gastrocnemius muscle Compared with the model control group,there was no significant effect in the passive stretch exercise group and the electroacupuncture intervention group(P>0.05),and the expression levels of the two proteins in the combined intervention group were significantly increased(P<0.05).Conclusions:(1)Electroacupuncture intervention can promote the recovery of the cross-sectional area of atrophic gastrocnemius muscle fibers,promote the increase of the proportion of slow muscle fibers,and achieve the purpose of improving muscle atrophy.(2)In contrast,the combination of passive stretch exercise and electroacupuncture can better promote the recovery of muscle cross-sectional area,and can resist the transformation of fast and slow muscle fibers。(3)the combination intervention can promote the expression of calacin troponin.Promotes the recovery of atrophic muscles.It is recommended that these two methods can be combined with interventional therapy in patients with treatment of disuse muscle atrophy.