| Objective: To understand the distribution of single nucleotide polymorphisms at three loci of rs7935564,rs1063303 and rs10838543 in tripartite motif protein 22(TRIM22)gene in our study population,and to further analyze the correlation of genetic polymorphisms at three loci of rs7935564,rs1063303 and rs10838543 with genetic susceptibility to cervical cancer.Methods: In this research,in cases of patients(a group of patients with cervical cancer)and control group(group of patients with cervical benign lesions and controls)to test research,using the snapshot sequencing method for all the research object of TRIM22 gene rs7935564,rs1063303,rs10838543 three loci sequencing,gene polymorphism and the Sanger sequencing method for sequencing.In order to test whether the object of this study conforms to the harden-weinberg equilibrium law of population genetics(HWE),the HWE test is carried out on the population included in this study to test whether the group representativeness and comparability of the object is reliable.The Odds ratio(OR)and 95% confidence interval(CI)were calculated to analyze the relationship between genetic polymorphism of 3 SNP loci and genetic susceptibility to cervical cancer after age correction by unconditional Logistic regression analysis.Results :1 Locus rs7935564 in TRIM22 gene:1.1 Locus rs7935564 in TRIM22 gene:(1)Genotype frequencies were as follows: GG(40.7%),GA(43.2%)and AA(16.1%)in cervical cancer group,GG(61.6%),GA(26.8%)and AA(11.6%)in benign cervical lesion group,GG(56.0%),GA(33.0%)and AA(11.0%)in control group.Statistical analysis showed that there was significant difference between cervical cancer group and benign cervical lesion group(P = 0.015).However,no obvious difference was found in the comparison of cervical cancer group(P = 0.120)or benign cervical lesion group(P = 0.611)with control group.1.2 Allele frequencies were described as follows in each group.: The frequency of G allele was 62.3%,75.0% and 72.5% in cervical cancer group,benign cervical lesion group and control group,respectively,and that of A allele was 37.7%,25.0% and 27.5% in the former three groups,respectively.Statistical analysis revealed that there were evident statistical differences in the comparison of cervical cancer group(P = 0.008)and benign cervical lesion group(P = 0.040)with control group,respectively;yet no statistical difference was detected between benign cervical lesion group and control group(P = 0.559).2 Locus rs1063303 in TRIM22 gene:2.1 The genotype frequencies were presented as follows in each group:GG(61.7%),GC(33.3%)and CC(5.0%)in cervical cancer group,GG(62.5%),GC(31.3%)and CC(6.2%)in benign cervical lesion group,GG(54.0%),GC(37.0%)and CC(9.0%)in control group.Through statistical analysis,it was discovered that there was no statistical difference between cervical cancer group and benign cervical lesion group(P = 0.900),between cervical cancer group and control group(P = 0.440),or between benign cervical lesion group and control group(P = 0.428).2.2 Allele frequencies were listed as follows in each group: The frequency of G allele was 78.4%,78.1% and 75.5% in cervical cancer group,benign cervical lesion group and control group,respectively,and that of A allele was 21.6%,21.9% and 24.5% in the above mentioned three groups,respectively.Subsequent statistical analysis revealed no obvious difference in the comparison between cervical cancer group and benign cervical lesion group(P = 0.949),between cervical cancer group and control group(P = 0.516),or between benign cervical lesion group and control group(P = 0.522).3 Locus rs10838543 in TRIM22 gene:3.1 The genotype frequencies were as follows in each group: TT(61.7%),TC(32.1%)and CC(6.2%)in cervical cancer group,TT(61.6%),TC(30.4%)and CC(8.0%)in benign cervical lesion group,TT(63.0%),TC(31.0%)and CC(6.0%)in control group.No statistical difference was indicated in the comparison between cervical cancer group and benign cervical lesion group(P =0.873),between cervical cancer group and control group(P = 0.945),or between benign cervical lesion group and control group(P = 0.847).3.2 Allele frequencies were as follows in each group: The frequency of T allele was 77.8%,76.8% and(78.5% in cervical cancer group,benign cervical lesion group and control group,respectively,and that of C allele was 22.2%,23.2% and 21.5% in the above mentioned three groups,respectively.Statistical analysis suggested no statistical difference in the comparison between cervical cancer group and benign cervical lesion group(P = 0.819),between cervical cancer group and control group(P = 0.869),or between benign cervical lesion group and control group(P = 0.673).4 TRIM22 gene genotypes and alleles were associated with the risk of cervical cancer and benign lesion4.1 TRIM22 gene genotypes and alleles at three sites were associated with risk of cervical cancer(1)GA comparison between rs7935564 cancer group and control group(P=0.101,OR=0.449,95%ci = 0.172-1.169);GA comparison between cancer group and benign group(P=0.026,OR=0.271,95%ci = 0.086-0.858);GA comparison between cancer group and non-cancer group(P=0.022,OR=0.364,95%ci = 0.153-0.866).There were differences between the cancer group and the benign group,but the risk of cervical cancer was not increased.A allele comparison between the cancer group and the control group(P=0.031,OR=0.609,95%CI= 0.388-0.956),A allele comparison between the cancer group and the benign group(P=0.006,OR=0.479,95%CI= 0.284-0.806),and A allele comparison between the cancer group and the non-cancer group(P=0.004,OR=0.548,95%CI= 0.364-0.825).There were differences between the cancer group and the control group,but no increased risk of cervical cancer.(2)Rs7935564 loci carcinoma group compared with non cancer two set of AA,rs1063303 loci carcinoma group and non cancer two GC,CC,C allele compared to parameters between groups,rs10838543 loci carcinoma and carcinoma group TC,CC,C allele compared to parameters between groups,the results show that all P > 0.05,carcinoma group compared with control group P values were 0.651,0.703,0.995,0.432,0.600,0.683,0.627,OR values were0.798,1.295,0.996,1.221,0.705,0.753,0.881,95%CI were 0.301 ~ 2.119,0.343 ~ 4.896,0.252 ~ 3.929,0.742 ~ 2.012,0.190 ~ 2.610,0.193 ~ 2.937,0.530 ~ 1.467,respectively.Comparison between the cancer group and the benign group showed that the P values were 0.346,0.846,0.932,0.787,0.738,0.615,0.956,OR values were 0.569,1.152,1.067,1.081,0.773,0.670,1.016,95%CI were 0.176 ~ 1.839,0.277 ~ 4.801,0.242 ~ 4.701,0.171 ~ 3.998,0.141~ 3.184,0.577 ~ 1.790,respectively.The results showed that there was no difference between the three groups in the mutation of AA gene at rs7935564,rs1063303 and rs10838543,which had no correlation with the risk of cervical cancer.4.2 TRIM22 gene genotype and allele at three sites were used to assess the risk of cervical benign lesions.Rs7935564 locus of GA,AA,A allele,rs1063303 locus of GC,CC,C allele,rs10838543 locus of TC,CC,C allele compared with the parameters of the control group and the benign lesion group,the results showed that all P>0.05,the difference was not statistically significant,the P values were 0.452,0.585,0.450,0.913,0.928,0.739,0.761,0.921,0.435,respectively.OR values were1.529,1.381,1.210,1.074,0.941,1.091,0.806,1.076,0.812,95%ci values were0.506 ~ 4.622,0.433 ~ 4.405,0.738 ~ 1.983,0.300 ~ 3.840,0.251 ~ 3.534,0.The results showed that the genetic mutations at rs7935564,rs1063303 and rs10838543 had no correlation with the risk of cervical benign lesions.Conclusion:The results of this research showed that:1.The TRIM22 gene rs7935564 genotype and allele frequency of cervical cancer group were significantly different from that of the benign lesion group,and the distribution of allele frequency of cervical cancer group was significantly different from that of the control group,the difference was statistically significan。2.TRIM22 gene rs7935564,rs1063303 and rs10838543 SNPS were not associated with the risk of cervical cancer. |
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