Y Chromosome Microdeletion Patients Were Treated With ART To Study Pregnancy Outcome

Infertility is the most common disease in male infertility.Its main cause is male testicular spermatogenic dysfunction.Its main manifestations are oligospermia,asthenospermia,malformed spermatozoa,etc.In recent years,the increasing incidence of oligozoospermia has led to more or less low fertility in more and more males,which is mainly manifested in the decline of semen quality,oligoasthenospermia and so on,thus seriously affecting reproductive health and population fertility [2,3].According to the existing research,about 10%-15% of couples with infertility in the world are caused by male factors,and spermatogenesis disorder caused by genetic defects is one of the important reasons affecting male fertility.Y chromosome microdeletion is the second most common genetic cause of male infertility,and about 7% of all infertile men have Y chromosome microdeletion.The development of intrafollicular sperm injection(ICSI)has greatly increased the possibility of oligospermia patients having their own children.Some studies have shown the relationship between Y chromosome microdeletions and clinical outcomes of assisted reproductive technology(ART).However,whether Y chromosome microdeletions have adverse effects on the outcome of assisted reproductive technology(ART)has not been clearly studied,and the results have been controversial.Although male infertility is a common health problem,it is important to understand its etiology and treatment methods,which is the inadequacy of current research on male infertility in China.In this study,we focused on the effects of assisted reproductive technology(ART)on pregnancy outcomes in oligospermia patients.Objective1.To understand the influence of genetic factors on male infertility,to improve the awareness of diagnosis and treatment of male infertility,and to improve the success rate of assisted reproductive technology(ART)in the treatment of male oligozoospermia,to provide scientific basis for clinical eugenics.2.To evaluate the fertility of oligospermia patients and the effect of assisted reproductive technology(ART)on pregnancy outcome.Materials and methods1.178 cases of male oligospermia undergoing assisted reproductive technology(ART)from June 2016 to June 2018 in the Third Affiliated Hospital of Zhengzhou University were selected as subjects.1)178 oligospermia patients were divided into group A(Y chromosome microdeletion)and group B(non-Y chromosome microdeletion)after physical examination,Y chromosome microdeletion and sex hormone detection.2)The subjects were followed up and the fertilization rate,cleavage rate,transplantation rate,clinical pregnancy rate,abortion rate,live birth rate and the ratio of male to infant were compared between A(n = 45)and B(n = 133)groups.3)According to the comparison of pregnancy outcomes between groups A and B,the assisted reproductive technology for oligozoospermia patients was evaluated comprehensively to provide reasonable suggestions for ensuring good birth and fertility of the population.Collect the case history of the study object,collate the clinical data of the study object,and analyze one by one.All the data were analyzed by SPSS21.0 statistical software.Firstly,the two groups of data were tested for normal distribution and orthodox distribution respectively,and then the two groups were compared one by one.Quantitative data expressed their average level and variation degree with x ±s.Independent sample t test was used for each group of data,and chi-square test was used for the comparison of qualitative data,and α =0.05 was used as the test level.2.The study meets the standards of the Hospital Ethics Committee and is approved by the Ethics Committee.The purpose and significance of this study will be detailed to inform patients,obtain informed consent of patients,and sign a written consent.Results1.A total of 178 oligospermia patients were enrolled,45 in group A(Y chromosome microdeletion)and 133 in group B(non-Y chromosome microdeletion).By comparing the general data of the two groups,it was found that there were no significant differences in the age of men,age of women,infertility years,BMI index of women and the number of eggs obtained(P > 0.05)(see Table 1).2.Physical examination of group A and group B showed that there was no significant difference in testicular volume between the two groups(P > 0.05).There was no significant difference between the semen volume and the PH value between the two groups(P > 0.05).The hormone level of men could reflect the spermatogenesis of male testis laterally.We stimulated the follicles of the two groups.There was no statistical significance between FSH,LH,T,PRL and E2(P > 0.05)(see Table 2).3.After ART,the fertilization rate of group A and B was 51% and 83%,respectively.Statistical analysis showed that there was statistical significance(P < 0.05);cleavage rate was 91.05% and 87.28%,respectively.Statistical analysis showed significant difference(P < 0.05);transplantation rate was 50% and 50.38%,clinical pregnancy rate was 38.88% and 37.40%,respectively.The yields were 5.55% and 4.38%,the live births were 30.55% and 25.95%,and the ratio of boys to infants was 45.4% and 58.8%,respectively.There was no significant difference between the two groups(P > 0.05)(see table 3).Conclusions1.The fertilization rate of non-Y chromosome microdeletion patients is higher than that of Y chromosome microdeletion patients after assisted reproductive technology(ART).2.Male offspring of Y chromosome microdeletion patients have inherited the genetic defects of their fathers,which are clinically infertile,while female offspring have no effect.