Discovery And Functional Studies Of Key Factors In Breast Cancer Based On Proteomics Analysis

Breast cancer is the most frequently diagnosed cancer globally and is the leading cause of cancer-related death in women.In recent years,the development of high-throughput proteomics technology has provided good technical support for the discovery of breast cancer biomarkers for early diagnosis,or the novel therapy targets.In this thesis,focusing on breast cancer progression,we performed LC-MS/MS proteomic strategy to search for the key factor which contribute significantly to the progress of breast cancer,and further carried out a biological experiment verification.Meanwhile,we conducted the functional research of LAPTM4B in vitro and in vivo.Finally,we found that LAPTM4B regulated the components in Akt/mTOR pathway to promote proliferation of MCF-7 cells via decreasing expression of p21 and Caspase-3,while promoted invasion,adhesion and angiogenesis via stimulating expression of HIF-la,MMP2 and MMP9.LAPTM4B may be a potential therapeutic target for breast cancer treatment.