| Objective: To investigate the expression of P170 protein in tumor tissues of patients with lung carcinoma and squamous cell carcinoma,and to determine the correlation between the expression of P170 protein in tumor tissues and prognosis and clinicopathological factors in patients with lung cancer and squamous cell carcinoma.The individualized treatment strategy and prognosis of patients with squamous cell carcinoma after operation provide the basis.Methods: From January 2013 to January 2016,544 patients with lung cancer were treated in the Department of Thoracic surgery from the first affiliated Hospital of Dalian Medical University.The follow-up methods were telephone follow-up and outpatient follow-up.The end date of follow-up was June 2018.The sex,age,mode of operation,scope of resection(partial lobectomy,lobectomy or partial lobectomy),pathological type,maximum diameter of tumor,degree of pathological differentiation,pleura invasion,Clinical data such as TNM(tumor node metastasis)staging and tumor-free survival time of the eighth edition of the International Union against Cancer.The expression of P170 protein in tumor tissue was detected by HE staining(hematoxylin-eosin staining,hematoxylin-eosin staining)and IHC(inmmun-ohistochemistry,immunohistochemistry).The expression of P170 protein in tumor tissues of 544 patients with lung cancer was counted and its relationship with clinicopathological features and prognosis was analyzed.Chi-square test and F test were used for counting data analysis,SPSS 21.0 was used for routine data follow-up analysis,survival data were drawn by Kapian-Meier,and univariate analysis was carried out by Log-Rank.The COX proportional risk regression model was used to correct the HR value and(95% CI),and it was statistically significant that the p value was less than 0.05.Result:Our study found that the expression of P170 protein is distributed in the cytoplasm or / and membrane of tumor cells,and its total expression rate is 44%(239/544),of which plasma expression accounts for 57.7%(139/239).Expression accounted for 31.0%(74/239)and serosal expression accounted for 11.3%(45/239).The expression of P170 protein was different in different pathological types,and the expression in adenocarcinoma accounted for 91.6%(219/239),which was significantly higher than other types of lung cancer.There was no significant difference in the expression of P170 protein between gender,age,pleural invasion,tumor differentiation and tumor maximum diameter(p>0.05).Univariate analysis found that the five-year survival time and gender of lung cancer patients(5-os% vs.76.1% vs 94.6%,p<0.001)and surgical approach(open thoracic surgery vs.thoracoscopic surgery 5-os%)67.1% vs 92.3%,p<0.001),P170 protein expression and expression site(P170 no expression vs plasma expression vs membrane expression vs serosal expression 5-os% was 85.7% vs 92.4% vs 82% vs 75.7 %,p=0.050),degree of pathological differentiation(differentiation vs differentiation,low differentiation 5-os%,93.9% vs 90.3% vs 75%,p=0.008),tumor maximum diameter(≤3 cm vs>3 cm 5-os There was a statistical difference in the percentage of 93.7% vs 49.1%,p<0.001,but no correlation with age,pleural invasion,and surgical resection(p>0.05).Multivariate analysis of postoperative survival time in patients with lung cancer found that women had a low risk of death(HR: 0.403 95% CI: 0.204-0.797,p=0.009);tumors with a maximum diameter > 3 cm had a high risk of death(HR: 3.495 95% CI: 1.838-6.644,p<0.001);P170 protein negative(-)(HR: 0.228,95% CI: 0.084-0.623,p=0.004)has a better prognosis than the P170 protein serosal expression,and the risk of death is low;The risk of death from thoracic surgery was high(HR: 2.491 95% CI: 1.307-4.748,p=0.006).The results showed that gender,tumor maximum diameter,P170 protein expression and surgical methods were independent risk factors affecting the prognosis of patients(p<0.05).Conclusions:1.Whether P170 protein is expressed and the relationship between the expression site and postoperative pathological type is higher in lung adenocarcinoma than in lung squamous cell carcinoma;2.Gender,tumor maximum diameter,P170 protein expression,and surgical methods are independent risk factors for the prognosis of patients with lung adenocarcinoma and squamous cell carcinoma. |
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