Expression Of Irisin In Liver Cancer And Its Effect On Hepatoma Cells

Objective and Significance:Hepatocellular carcinoma(HCC)is one of the most common and most malignant malignant tumors in the world.The incidence and mortality of liver cancer in China is the highest in Asia.Irisin is a newly discovered muscle factor and adipokines formed by proteolytic hydrolysis of type III fibronectin domain 5(FNDC5),which has the function of browning white adipose tissue and improving energy expenditure.Studies have shown that irisin is abnormally expressed in a variety of tumors and is important for the occurrence and development of tumors,including breast cancer,lung cancer and osteosarcoma,however,the expression of irisin in liver cancer and its influence on liver cancer have rarely been reported.In this paper,we examined the expression levels of irisin in 20 pairs of hepatocarcinoma tissues and adjacent tissues,and studied its effects on liver cancer cells,in order to provide new ideas for early diagnosis and treatment of liver cancer.Objective: To determine whether there is a difference in the expression of irisin in HCC tissues and adjacent tissues,serum of HCC patients and serum of healthy volunteers.To investigate the effects of irisin on the proliferation,apoptosis and migration of hepatoma cells He PG2.Methods:1.The expression of Irisin protein in liver cancer and its adjacent tissues was detected by RT-PCR in 20 cases of liver cancer and its adjacent tissues.2.The expression levels of irisin in HCC cell line HePG2 and normal liver cell line LO2 were detected by RT-PCR.3.Serum levels of irisin in 20 patients with liver cancer and 15 healthy volunteers were detected by enzyme-linked immunosorbent assay(ELISA).4.The effects of different concentrations of Irisin(0.625 n M,1.25 n M,2.5 n M,5 n M,10 n M,20 n M)cultured for different time(12h,24 h,36h,48 h,72h)on the proliferation of HCC cells were detected by CCK-8 assay.5.The effect of Irisin on the migration ability of HCC cells after cell scratch assay.6.Effect of Irisin-indused cells on apoptosis and cell cycle of HCC cells by flow cytometry.Result:1.The expression level of irisin in HCC tissues and cell line were significantly higher than that in the control groups.2.There was no significant difference in the expression of irisin in the serum of HCC patients and healthy volunteers.3.The results of CCK8 showed that different concentrations of Irisin could promote the proliferation of hepatoma cells for a certain period of time.The concentration of Irisin at 2.5 n M for 24 h could furthest promote the proliferation of HCC cells.4.Cell scratch test results show that Irisin can significantly increase the migration ability of liver cancer cells after acting on liver cancer cells.5.Flow cytometry results showed that the number of apoptosis of liver cancer cells decreased after Irisin treatmented.Cell cycle arrest increased in S/G2 phase,and G0/G1 phase decreased significantly,further explanation that Irisin can promote liver cancer cell proliferation and inhibit its apoptosis.Conclusion:1.The expression level of irisin in HCC tissues and cell lines were significantly higher than that in the control groups,and the difference was statistically significant.2.Irisin promotes proliferation,migration and inhibits apoptosis of HCC cells.Summary: The expression level of irisin in HCC tissues and cell line wre higher than that in the control groups.There is no significant difference in the expression level of serum in liver cancer patients and healthy volunteers,which may be related to the form change of irisin in the blood.In addition,the irisin can significantly promote the proliferation of HCC cells,increase the migration ability and inhibit the apoptosis of HCC cells at the cellular level.This feature may provide new ideas for the early diagnosis and treatment of liver cancer.