A Study On The Changes Of Plasma Heat Shock Protein 90α Expression In Breast Cancer Pre- And Post- Neoadjuvant Chemotherapy

Objective:To investigate the relationship between the changes of plasma HSP90αexpression pre-and post-neoadjuvant chemotherapy and the efficacy of neoadjuvant chemotherapy in breast cancer.Methods:Plasma HSP90αwas detected by ELISA method in 79 cases of breast cancer,30 cases of benign breast tumors and 17 cases of non-neoplastic breast lesions.Plasma HSP90αwas detected after neoadjuvant chemotherapy and after operation in breast cancer.To analyze the difference of plasma HSP90αexpression in different breast lesions and the relationship between plasma HSP90αexpression and clinicopathological features of breast cancer.To analyze the changes of plasma HSP90αexpression pre-and post-neoadjuvant chemotherapy and pre-and post-operation in breast cancer.The data were processed by SPSS 23.0 software.The ROC was drawn by MedCalc 18.11.3 software to evaluate the relationship between plasma HSP90αexpression changes pre-and post-chemotherapy and the efficacy of neoadjuvant chemotherapy.Results:The plasma HSP90αexpression in breast cancer is significantly higher than that in benign breast tumors and non-neoplastic lesions(χ~2=28.163,P<0.0001),and is associated with histological grade(χ~2=18.570,P<0.0001).After neoadjuvant chemotherapy,plasma HSP90αexpression increased in PD group(Z=-2.201,P=0.028),decreased in PR group(Z=-3.307,P=0.001),and there is no significant change of plasma HSP90αexpression in SD group(t=1.214,P=0.2397).After neoadjuvant chemotherapy,plasma HSP90αexpression increased in G1 group(Z=-2.197,P=0.028),decreased in G3 group(Z=-2.346,P=0.019),and there is no significant change in plasma HSP90αin G2group(t=1.397,P=0.1816).Plasma HSP90αexpression was not declined significantly in G4-5 group(t=2.143,P=0.0987).The AUC ofΔHSP90α%which evaluating the effect of chemotherapy is 0.785(95%CI:0.666~0.876 RECIST1.1)and 0.801(95%CI:0.664~0.901 Miller-Payne),and the Cutoff value is 16.63%.Conclusion:Plasma HSP90αexpression in breast cancer can reflect tumor load,and its changes before and after neoadjuvant chemotherapy can effectively reflect the sensitivity of the tumor to chemotherapy.