A Study On Interventional Mechanism Of Replenishing Qi And Resolving Fluid Retention Formula On Asthma Rats With Syndrome Of Cold Fluid Accumulation In Lung Based On The Correlation Between Autophagy And Lung-Qi Deficiency

Objective: The thesis aims to explore the correlation between lung qi deficiency and autophagy,and replenishing qi and resolving fluid retention formula is adopted to intervene asthma rats with syndrome of cold fluid accumulation in lung,observing its interventional mechanism on autophagy in the lung tissue.Methods: Both theoretical and experimental methods are employed in the study.(1)Theoretical study: based on Canon of Medicine and other medical classics as well as xiangthinking,the paper studies the concept and its function systematically and probes into the influence of lung qi deficiency on autophagy in the process of information transmission.(2)Experimental study: we establish bronchial asthma rat model with syndrome of cold fluid accumulation in lung by sensitization with ovalbumin,freezing in fridge,raising with cold water,and overwork with swimming.Rapamycin,Xiaoqinglong decoction,warming yang and resolving fluid retention formula and Huangqi(milkvetch root)are used respectively for treatment in each group,and we observe the relevant indexes.Results: The rat model is successfully established.The signs and symptoms of rats correspond to syndrome of cold fluid accumulation in lung,which also manifest in micro indexes,including lung function,pathological section and inflammatory factors.The abovementioned four ways of treatment are effective in different ways,among which replenishing qi and resolving fluid retention formula is most effective.Conclusion: Qi,as the manifestation of information transmission,is related to autophagy.Lung qi can influence the information transmission in the process of autophagy by regulating qi of the body,and thus lung qi deficiency leads to the disharmony of autophagy,influencing the process of qi transformation and its protective function.In the process of disease onset,lung qi deficiency leads to the disorder of qi transformation,manifesting as increased secretion of inflammatory factors,programmed cell death and over-accumulation of pathological product,such as phlegm.Hence,replenishing qi and resolving fluid retention formula is effective in the treatment of syndrome of cold fluid accumulation in lung.Its therapeutic mechanism may lie in restraining the signaling pathways expression of mTOR,increasing the Atg to improve autophagy activity and reduce airway inflammation.