Effect Of CDK5 On B16 Migration And Tyrosinase Activity Of Mouse Melanoma Cells

Melanoma is the most aggressive malignant cancer that occurs in the skin.It is characterized by high mortality and recurrence after treatment.In the biology of melanoma cells,the inhibition of cell proliferation,differentiation and migration are of great significance for the control and treatment of melanoma.It has been found that Cdk5 is highly expressed in melanoma cells,and cdk5-knockdown can lead to apoptosis and death of cells.However,the migration of melanoma cells and the activity of tyrosinase that leads to produce melanins have not been reported.In this research,the effect of cdk5-knockdown on the migration of B16 melanoma cells and the activity of tyrosinase were investigated in Cdk5 knockdown B16 cells.Real-time quantitative PCR and Western blotting results showed that the expression of integrin betal,integrin beta5 and PDGF-B could be inhibited by Cdk5 knockdown both at transcriptional and translational level in B16 melanoma cells.The cell scratch test showed that Cdk5 knockdown could inhibit the migration of B16 melanoma cells.The results of tyrosinase activity assay showed that the Cdk5 knockdown in B16 melanoma cells inhibited the activity of tyrosinase.The results suggested that Cdk5 could inhibit the migration of B16 melanoma cells by regulating the expression of integrin betal,integrin beta5 and PDGF-B and regulate the activity of tyrosinase in B16 cells.