The Expression Of SORD In Clear Cell Renal Cell Carcinoma And Its Effect On Proliferation And Migration Of 786-O Kidney Cancer Cells

Objective: To investigate the relationship between SORD expression and clear cell renal cell carcinoma and its effect on proliferation and migration of786-O cell line.Methods: Firstly,the expression of SORD in 50 cases of clear cell renal cell carcinoma and adjacent normal tissues resected from urinary surgery in our hospital was confirmed by immunohistochemistry.Next,SORD mRNA expression in tissues was detected by real-time quantitative PCR,and the clinical correlation between SORD mRNA expression level and patient ages,genders,tumor sizes,and pathological stages were further analyzed.Then the SORD stably over-expressed 786-O cells were constructed.The cellular proliferation abilities were then observed by WST-1,clone formation and BrdU,and the cell migration abilities were detected by scratch test and Transwell assay.Results:1.The decline of SORD expression in clear cell renal cell carcinoma:Immunohistochemistry results showed that the SORD protein expression was significantly lower in clear cell renal cell carcinoma than in adjacent normal renal tissues;real-time quantitative PCR was used to detect SORD mRNA expression.Consistent with the results of immunohistochemistry,paired t-test was used for statistical analysis,P<0.0001 represented statistically significant;clinical data analysis showed that there was no significant correlations between SORD mRNA expression and genders,tumor sizes,clinical symptoms,and clinical pathological staging(p >0.05),but correlated with ages(p<0.05).2.SORD reduced 786-O cell proliferation: the absorbances of 786-O,786O-NC and 78SO-SORD for 24 h,48h and 72 h detected by WST-1 assay were0.16±0.004,0.17±0.003,0.16±0.062,and 0.87±0.024.0.87±0.003,0.56±0.007,and1.35±0.053,1.35±0.032,0.84±0.006,respectively.The results showed that SORD inhibited the growth of 786-O cells(P<0.01);Clone formation experiments were conducted when the planting density was 300.The number of colonies formed by786-O,786O-NC and 7886-0-SORD was 106.5±4.95,109.5±6.36 and 80.5±0.71,respectively;when planting density was 500/well,they were 118.5±2.12 and108.5±10.61 respectively.83±2.83;Both planting densities showed that SORD reduced the number of cell clones(P<0.05;P<0.01);in BrdU experiments,the proportion of proliferating cells was(66.47±2.45)% and(65.76±1.77)%respectively.(44.39±1.77)%.786O-NC compared with control cells,there was no statistically significant difference,but the proliferation of 7886-0-SORD group was lower than that of control group and empty group(P<0.01,P<0.001).3.SORD reduced the migration ability of 786-O cells: The relative mobility of786-O,786O-NC and 786O-SORD groups after scratching for 24 h were0.55±0.02,0.58±0.05,and 0.39±0.04,respectively.Compared with the empty group,the results showed that SORD decreased the relative migration rate of786-O cells(P<0.01);Transwell experiment,the number of cells passing through the small chamber after 24 hours was 134.67±12.22,138.33±8.08 and 102±10.58,respectively.There was no significant difference in the migration ability of the control group,but the migration distance was shorter in the 786O-SORD group compared with the control group(P<0.05),and the migration ability was decreased compared with the empty group(p<0.01).Conclusions: The expression of SORD was decreased in clear cell renal cell carcinoma;overexpression of SORD can inhibit the proliferation and migration of 786-O clear cell renal cell carcinoma cell line.