| Objective:This study simulated OSAS to establish a rat model of CIH by measuring the expression of autophagy-related proteins LC3 II and Beclin-1 in rat neural cells to investigate the effect of CIH on autophagy and its clinical significance,and to further explore endogenous if the cannabinoid systems（ECs）affect the changes in autophagy.Methods:40 healthy male rats were selected to simulate OSAS to establish a CIH model and randomly divided into 5 groups:normal control group,IH group,and IH+intervention group.IH group was divided into IH4 week group and IH6 week group.IH+intervention group was divided into 4 weeks of IH+intervention and 6 weeks of IH+intervention.The intervention group received an intraperitoneal injection of CB1 antagonist（Rimonabant）1.5 mg/kg/d before modeling.At the 4th week and the 6th week of the experiment,half of the rats were randomly selected from each group.The brain tissues of each group were then isolated.The morphological changes of neurons after HE staining were observed with an optical microscope.The expression of autophagy-related proteins LC3 II and Beclin-1 in hippocampus of rats was detected by immunohistochemistry（SABC method）.Results:1.Pathological changes of HE staining:nerve cells in the control group were regular and normal.In the 4th week,neurons in the IH group were damaged,arranged loosely,the cytoplasm was sparse,and the edges of the cells were not clearly observed under the microscope;6 weeks IH The number of nerve cells in the group gradually decreased,and the arrangement was disordered.The cytoplasm was particularly sparse;the nerve cells in the 4-week hypoxic+intervention group were more neatly arranged and increased in number compared to the hypoxic 4-week group,and the periphery of the cell boundary was observable under the microscope.Compared with the hypoxic 6-week group,the number of neurons in the hypoxia+intervention group was increased,but the cytoplasm was sparse and disordered.2.Compared with the control group,the expression of LC3II and Beclin-1 protein in hippocampus of IH rats was significantly increased,and the difference was statistically significant（P<0.05）.Among them,IH group had the most obvious increase in 4 weeks.3.Compared with IH alone group,the expression of LC3II and Beclin-1 protein in the hippocampus of the intervention group was significantly decreased,and the difference was statistically significant（P<0.05）.Among them,the decrease was more obvious in the 4-week intervention group..Conclusion:1.It was confirmed that CIH could induce the autophagy of rat neural cells,but with the prolonged interval time,the expression of autophagy gradually decreased;2.CB1 antagonist（rimonabant）intervention can inhibit ECS disorders caused by CIH,thereby reducing the occurrence of CIH-induced autophagy in rat neurons. |
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