Variation In Expression Levels Of Zip13 In Heart Failure With Preserved Ejection Fraction Rat Heart And Its Mechanism Of Action

ObjectiveTo establish a model of heart failure with preserved ejection fraction(HFpEF)in rats and to measure variation in zinc contents and expression levels of Zip13 in myocardial of HFpEF rats.Besides,To investigate the role of zinc and Zip13 in myocardial remodeling of HFpEF and its possible mechanism.MethodsIn order to establish HFpEF rat model,we choose Dahl salt-sensitive(DSS)rats and fed a high salt diet to experimental group.However,the control group was fed a normal salt diet.The content of sodium chloride in high salt feed was 8%,while the normal diet group was 0.3%.The remaining feed ingredients and feeding conditions are same.Blood pressure and heart rate were measured by the noninvasive blood pressure detector every two weeks.In 6th week,12 th week and 19 th week respectively,the rats were detected by animal specific ultrasound.To detect variation in zinc contents and expression levels of Zip13 in myo-cardial of HFpEF rats,The left ventricular myocardium specimens were extracted from the successful HFpEF model rat and the control group of corresponding time.Immediately myocardial specimens stored at-80℃until use.The zinc levels of specimens were measured by Inductively Coupled Plasma Optical Emission Spectroscopy(ICPOES).RT-fq-PCR and Western blot were used to detect mRNA and protein expression of Zip13 in the myocardium.ResultsThe HFpEF animal model made by DSS rats feeding for a high salt diet meet the design requirements.zinc contents in rat heart tissue was significantly increased in HFpEF group when compared to control group.In terms of cardiac morphology,the model group showed significant hypertrophy compared with the control group.the mean systolic blood pressure(SBP),LVM and LVM/W in HS-group was significantly higher than that in the NS-group but did not,however,show a significant difference with EF in 19 th weeks.Compared to the control group,Zip13 mRNA and protein expression levels in the HFpEF rat heart were markedly increased.Conclusion:The concentration of zinc ion in myocardium of HFpEF rat was elevated,that is,there was a steady Zinc homeostasis imbalance in myocardium.In conclusion,zinc accumulation of the HFpEF rat heart were correlated with up-regulation of Zip13 expression.However,the exact mechanism of these interactions needs to be further investigated.