Expression Of MicroRNA31-5p In Colorectal Cancer And Its Clinical Significance

Background: Colorectal cancer is a common malignant tumor in the gastrointestinal tract.Its early symptoms are not obvious.It is a malignant tumor that seriously threatens human health.Mechanisms of colorectal tumorigenesis have been extensively studied at the molecular level and recently entered the field of micro RNAs.Micro RNAs(mi RNAs)are a class of small RNAs of about 22 nucleotides in length in eukaryotes,similar to small interfering RNAs(si RNAs),which regulate gene expression after transcription and control various cellular mechanisms involved in post-transcriptional gene transcription Regulation,the research group early use of subtractive Cell-SELEX technology to obtain specific binding with metastatic colorectal cancer aptamer W3,the use of flow cytometry on the target cell Lo Vo W3 binding specificity of cell sorting,collected W3 high and W3 low cell subpopulations.The two cell subpopulations were subjected to micro RNA expression profiling to obtain a group of differentially expressed micro RNA molecules.After verification,we selected mi RNA31-5p for further study.Objective: To investigate the expression of micro RNA-31-5p in colorectal carcinoma and its effect on the growth of colorectal cancer cells,and to analyze its clinical relevance.Mi RNA inhabitor transfection to reduce the expression of mi RNA in lovo cells,observe the proliferation,invasion and migration of lovo cells,analyze the biological role of mi RNA-31-5p in colorectal cancer,and further analyze the potential target genes for colorectal cancer Of gene-targeted therapy to lay the foundation.Methods: Forty fresh colorectal carcinomas and normal colorectal tissues were collected.The expression of micro RNA-31-5p in 40 colorectal cancer tissues and adjacent normal tissues was detected by SYBR GREEN real-time quantitative PCR.The micro RNA-31-5p expression levels and clinical pathological stage parameters such as correlation.In lovo,sw620,HTC-8 and HT-29,lovo was screened for mi RNA-31-5p overexpression using q RT-PCR technique.Lipofectamine 3000 was used as a vector to transfect mi RNA inhabitor into Lo Vo cells After the expression of mi RNA-31-5p was low,the effect of mi RNA-31-5p on the proliferation of colorectal cancer cells was detected by MTT assay.The effect of micro RNA-31-5p on migration and invasion of colorectal cancer cells was detected by Transwell assay.Results: The expression level of Micro RNA-31-5p in 33 colorectal cancer tissues was significantly higher than that in adjacent non-cancerous tissues(P <0.001).The expression of mi RNA-31-5p in colorectal cancer tissues was significantly correlated with TNM Staging was positively correlated with regional lymph node metastasis was positively correlated.mi RNA-31-5p was expressed to different degrees in all four kinds of colon cancer cells,and was the highest in lovo.The results of micro RNA-31-5p transfected with Lo Vo cells and untransfected MTT showed that micro RNA-31-5p promoted the proliferation of colorectal cancer cells.Transwell and scratch assay showed that micro RNA-31-5p promoted the migration and invasion of cancer cells PromoteConclusion: The study confirmed that micro RNA-31-5p is associated with the occurrence and development of colorectal cancer and has a markedly high response in colorectal cancer,which may serve as a new potential biomarker for the diagnosis and treatment of colorectal cancer.The proliferation of Lo Vo cells transfected with inhabitor mi RNA-31-5p decreased,and the invasion and metastasis decreased.In summary,mi RNA-31-5p may play an important role in the development of colorectal cancer.Differential expression of specific micro RNAs can help distinguish colorectal cancer from other colorectal diseases,and can be used as a reference to evaluate the prognosis of patients with different chemotherapeutic drugs.