Effect Of Human Peripheral Blood Progenitor Derived Mast Cells On The Growth Of Human Bronchial Epithelial Cells

Objective:To study the effect of human mast cells on the growth of bronchial epithelial cells.Methods:The progenitor cells(mast cell precursor cells)were isolated and purified from human peripheral blood and were induced differentiation into mast cells in vitro.The immunohistochemical method and the Western blot method were used to detect the expression of CK-8,SMA,E-cad and Vimentin in 16 HBE cell after co-culture with mast cells for 3 days.The effect of mast cells on the migration ability of 16 HBE cells was detected by the wound healing test.The effect of mast cells on the proliferation of 16 HBE cells was detected by the CCK-8 method.Results:1.The cultured cells identified by May-Gruenwald Giemsa staining method and the immunohistochemical staining method were confirmed to be the human mast cells.2.The results of immunohistochemistry demonstrated that human peripheral blood progenitor cell-derived mast cells could enhance the expression of Vimentin and α-SMA protein in 16 HBE cells,reduce CK-8 and E-Cad expression.The western blot results demonstrated that mast cells could decrease the expression of CK-8 and E-cad in 16 HBE cells and enhance the expression of Vimentin and SMA protein.3.The wound healing test results demonstrated that human peripheral blood progenitor cell mast cells can promote the migration of 16 HBE cells.The CCK-8 results demonstrated that the mast cells can promote the proliferation of 16 HBE cells.Conclusion:Human peripheral blood progenitor cell mast cells can enhance the expression of Vimentin and α-SMA protein in 16 HBE cells,decrease the expression of CK-8 and E-cad,and promote the migration and proliferation of epithelial cells.The results suggest that the epithelial mesenchymal transition(EMT)might be observed during the co-culture of the human bronchial epithelial cells with the mast cells.