| Objective:Study on the effect of VIP with different concentrationon CD11 c,CD40,CD80,CD86,TOLL receptor expression of DC and cell toxic of VIP on DC.Thus clear the regulating mechanism of VIP on DC activation to provide the basis for later experiment.Methods:1.Detect DC cytotoxicity with CCK8 method.2.Detect the changes ofexpression on CD11 c,CD40,CD80 and CD86 of DC by flow cytometry.3.Detect the the changes of expression on TLR2 and TLR4 receptors by RT-PCR.Results:1.According the results of experiment,we found that VIP with different concentration from 0.1nM(10-10m)/L to 100uM(10-4m)/L had no obvious toxicity on DC after 12,24,48,and 72 h.2.VIP had the ability of stimulating the expression of surface molecules on DC,especially toward CD86.3.VIP inhibited the expression of TLR2 and TLR4 receptorssignificantly,and no difference of the effect was found between TLR4 receptor and TLR2 receptor.Theinhibitory effect was correlatedpositively with time and dose.Conclusion:VIP can not stimulate the expression of CD40,CD80,CD86,CD11 ceffectively but inhibit the expression of TLR2 and TLR4 receptor in working concentration range,thus achieving the regulation of DC. |
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