Study On The Relationship Between Clinicopathological Features And EGFR And KRAS Gene Mutations In Lung Adenocarcinoma

Objective:In this study,we retrospectively studied the data of some patients with adenocarcinoma of the lung,analyzed the relationship between general clinical data,different pathological subtypes and mutations of EGFR and KRAS genes,and analysis of the relationship between general clinical data and the abundance of EGFR mutations provides a new perspective for follow-up exploration.Methods:Collected 66 patients with early operable lung adenocarcinoma admitted to the Fourth Hospital of Hebei Medical University from May 2014 to October 2016.The clinical stage is IA-IIIB,and the next-generation sequencing(NGS)is applied to record the mutation of lung cancer-related driver genes in 66 patients.The data obtained from the experiment was input into SPSS 21.0statistical analysis software,and the clinicopathological features such as sex,age,stage,smoking history,gene mutation type,postoperative pathological subtype classification,and gene mutation abundance were assigned;Comparison of gene mutations between different clinical characteristics groups using the four-table test or the Fisher exact test;The tumor stage of the different mutation groups was compared using the Wilcoxon rank sum test.P values less than 0.05 was considered statistically significant.Results:1.The relationship between clinicopathological features and mutations of EGFR and KRAS genes1)EGFR gene mutations in NSCLC patients: The mutation rate in female patients was higher than that in males(P<0.05),and the difference was statistically significant;the non-smokers mutation rate was higher than that insmokers(P<0.05).The difference was statistically significant;different ages There was no significant difference in the rate of EGFR gene mutation between patients(P=0.770);the incidence of EGFR gene mutation was not statistically significant during different tumor stages(Z=-0.916,P=0.359).2)KRAS gene mutations in NSCLC patients: The mutation rate in female patients was lower than that in male patients,but the difference was not statistically significant(P=0.412).The mutation rate in smokers was higher than that in non-smokers(P<0.05).The difference was statistically significant.There was no significant difference in age and KRAS mutation(P=0.125).The mutation rate was 26.1%.There was no significant difference between KRAS gene mutation and tumor stage(Z=-0.220,P=0.826).2.The relationship between pathological subtypes and EGFR gene mutations.The most common subtypes in this study were acinar predominant,followed by solid predominant papillary predominant,and lepidic predominant,invasive mucinous adenocarcinoma.EGFR gene detection results showed that acinar predominant adenocarcinomas had the highest mutation rate of EGFR,followed by papillary predominant,and lepidic predominant,solid predominant,invasive mucinous adenocarcinomas have no EGFR mutations.The rate of EGFR gene mutation among different adenocarcinoma subtypes was statistically significant(P<0.05).3.Relationship between abundance of EGFR gene and clinical characteristics.Take abrupt abundance of 20% as cutoff value;There was no significant difference in the abundance of EGFR mutation with sex(P=0.0.246),age(P=0.453)and smoking history(P=1.00);There was no significant difference in the abundance of EGFR mutations and tumor stage(Z=-0.500,P=0.617).Conclusions:1.In patients with lung adenocarcinoma,EGFR genes are more susceptible to mutations in women and non-smokers;KRAS genes are more susceptible to mutations in patients with a history of smoking;KRAS mutationrate in male patients is higher than that in women;EGFR and KRAS gene mutations There is no obvious correlation between status and tumor stage.2.There were significant differences in EGFR mutations rate and different pathological subtypes of adenocarcinoma.The frequency of EGFR mutations was higher in acinar predominant adenocarcinomas and papillary predominant adenocarcinomas,and lower in invasive mucinous adenocarcinomas.3.In lung adenocarcinoma,the abundance of EGFR gene mutations was not significantly associated with age,sex,smoking history,and tumor stage.