MicroRNA-27a Expression In Tongue Squamous Cell Carcinoma And Its Effect On Proliferation And Apoptosis Of Tongue Squamous Cell Carcinoma Cal-27 Cells

Objective : Tongue squamous cell carcinoma(TSCC)is one of the common malignant tumors in oral and maxillofacial region,which is characterized by high malignancy,high local recurrence rate and early cervical lymph node metastasis.In clinic,patients with TSCC are treated mainly by surgery,supplemented by radiotherapy,chemotherapy or targeted therapy.The tongue is an important functional organ,most of the patients in the middle and late stage who after radical surgery often cause speech,chewing,and other aspects of functional disorders.Therefore,"early diagnosis,early treatment" has gradually aroused the general attention of clinicians.Furthermore,recent research focuses on the screening of abnormal expression genes in TSCC to help in the early detection and adjuvant therapy of TSCC.MicroRNAs are a class of non-encoded single strand RNA molecules,which is approximately 20~25 nt,and is completely or incompletely paired with the target Gene 3 ’ End coding region,which causes the target gene to silence or block the target gene translation process to regulate the gene expression activity.Causes the cell biological behavior to change or causes the occurrence and the development of certain diseases.Consequently,this study is intended to investigate the expression of microrna-27 a in TSCC by molecular and cell biology methods,and to analyze its effects on the proliferation and apoptosis of TSCC cells.Methods:From September 2015 to September 2017,the tumor tissue specimens and corresponding paracancerous tissues of 35 patients with TSCC with complete medical records were collected from the Fourth Hospital of Hebei Medical University.Secondly,RT-qPCR was used to detect the expression of microRNA-27 a in TSCC tissues,corresponding paracanceroustissues,TSCC cal-27 cells and human normal tissue derived hek-293 cells.Thirdly,the Liposome transfection method was used to transfer microrna-27 a inhibitor into cal-27 cells of TSCC.The effects of microrna-27 a on cell viability and apoptosis of TSCC were further detected by MTS and flow cytometry(Annexin V-FITC(14)PI).The experimental data using SPSS 21.0software for statistical analysis,with p<0.05 as the difference is statistically significant.Results : The RT-qPCR results showed that the expression of microrna-27 a in 35 patients with TSCC was 2.81±3.17 times of the adjacent tissues,and the difference was statistically significant(p<0.05).The expression level of mir-27 a in cal-27 cells of TSCC was higher than normal hek-293 cells,and the difference was statistically significant(p<0.05).The expression of microrna-27 a in cal-27 cells of TSCC was lowered by transfection of microrna-27 a inhibitor,and the difference was statistically significant(p<0.05).The results of MTS and apoptosis showed that transfection of microrna-27 a inhibitor group and microrna-27 a inhibitor negative control group and mock group,the differences were statistically significant(P <0.05).Conclusion : MicroRNA-27 a is upregulated in tongue squamous cell carcinoma.The expression of microrna-27 a in TSCC has the function of promoting cell proliferation and inhibiting apoptosis,which may play a role in the development of TSCC.Microrna-27 a has the potential value to be a new diagnosis and treatment target,which can help the early diagnosis of TSCC and improve the comprehensive treatment scheme for patients.