Study On The Processing Technology And The Quality Of Liquorice Processed Pheretima Aspegillum

ObjectiveStudy on the processing technology of the Liquorice Processed Pheretima Aspegillum,find out and optimize single factors.Then the orthogonal test was designed to examine the processing technology of the Liquorice Processed Pheretima Aspegillum,and the better technology was gained.Meanwhile,we study on the quality of 11 batches of the Liquorice Processed Pheretima Aspegillum and the quality standard were based on the optimized technology.Then the quality standard would be drafted to reveal the meaning deeply about the Liquorice Processed Pheretima Aspegillum.Finally,to study the HPLC characteristic chromatogram of the Pheretima Aspegillum and the Liquorice Processed Pheretima Aspegillum,the common peaks information were obtained,and the HPLC characteristic chromatogram would be established.MethodsWe used the content of hypoxanthine,inosine and water-soluble extractives as indexs,and explored single factor test including the liquorice dosage,decoction time,the water quantity,soaking time and the dryingtemperature.And the orthogonal test was designed to examine the processing technology of the Liquorice Processed Pheretima Aspegillum.Pefer to 2015 edition“Chinese pharmacopoeia”,we determined to identify the Liquorice Processed Pheretima Aspegillum.The identification included appearances,microscopy,TCL,the moisture content,the total ash,the acid-insoluble ash,water-soluble extractives,and added identification of the content of hypoxanthine and inosine.Meanwhile,we analyzed the qualities of different batches of the Liquorice Processed Pheretima Aspegillum.Finally the quality standard of the Liquorice Processed Pheretima Aspegillum would be drafted by the experimental results.Finally,the fingerprints of the Liquorice Processed Pheretima Aspegillum would be established by HPLC with the method of using inosine as the reference peak,and the common peaks information were obtained.The similarity analysis of common peaks information were carried out by using the similarity degree evaluation system of traditional Chinese medicine fingerprint similarity evaluation system.And the HPLC characteristic chromatogram of the Pheretima Aspegillum and the Liquorice Processed Pheretima Aspegillum would be established.ResultsStudy on the processing technology of the Liquorice Processed Pheretima Aspegillum,optimize three single factors which are the Liquorice’s quantity,the soaking time and the drying temperature.And the orthogonal test was designed to examine the processing technology of the Liquorice Processed Pheretima Aspegillum.The results showed that the best processing technology of the Liquorice Processed Pheretima Aspegillum was A₁B₁C₃,that was to say the Liquorice’s quantity was15%,the soaking time was 20 min and the drying temperature was 70℃.Then,we analyze the qualities of different batches of the Liquorice Processed Pheretima Aspegillumbyidentificationinappearances,microscopy,TCL,themoisture content,the total ash,the acid-insoluble ash,the content of water-solute,the content of hypoxanthine and inosine.Finally the quality standard of the Liquorice Processed Pheretima Aspegillum is drafted.The fingerprints of the Pheretima Aspegillum and its products were established.There were 20 peaks of HPLC fingerprints of the Pheretima Aspegillum and 21 peaks of HPLC fingerprints of the Liquorice Processed Pheretima Aspegillum.And HPLC fingerprints were analyzed and compared.The similarity of map shows that there are some differences in different batches.The content of some components is different,but the composition is basically same.ConclusionTo optimize and standardize the processing technology indicators of the Liquorice Processed Pheretima Aspegillum,the better technology was gained.The quality standard of the Liquorice Processed Pheretima Aspegillum is drafted as follows:it ought to conforms the demand of identification in appearances;the Leucine,Lysine,Valine and Glycyrrhetnic acid appear in the corresponding position in TCL;the moisture content cannot exceed 10.0%;the total ash cannot exceed 10.0%;the acid-insoluble ash cannot exceed 5.0%;the content of water-solute extract cannot lower than 16.0%;the content of hypoxanthine cannot lower than 0.06%;the content of inosine cannot lower than 0.15%.The HPLC method had the advantages of high sensitivity and strong specificity.HPLC fingerprint and the content of inosine can fully reflect its intrinsic quality and provide scientific basis for the quality control of the Liquorice Processed Pheretima Aspegillum.