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Based On Animal Welfare Physical Factors On Miniature Pig Blood And Pig Blood And Biochemical Indicators And Anti-stress Effects Of Ginsenosides

Posted on:2019-10-22Degree:MasterType:Thesis
Country:ChinaCandidate:X X GaoFull Text:PDF
GTID:2404330548985505Subject:Integrative basis
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Objective Based on the study of experimental animal welfare and the monitoring of ion biochemical indexes and blood routine indexes of ordinary miniature pigs,the stress response of miniature pigs under noise and road transportation and the anti-stress effects of ginsenosides were studied.Experimental animals provide the basis for the daily adaptive feeding and use.Methods Through the noise and transportation,observe the ion biochemical indexes and blood routine indexes to study the stress response of ordinary miniature pigs under different conditions and the anti-stress effects of ginsenosides.Effect of noise on ion biochemical indexes and blood routine indexes of ordinary miniature pigs and the anti-stress effects of ginsenosides.The national standard GB14925-2010 as a reference,using noise(60d B,100 d B)and time(2h,4h,6h)two design points,Twelve ordinary miniature pigs were divided into two groups(ginseng total saponin group and blank group),6 in each group,One hour before the experiment,ginseng total saponin was given to the treatment group,and the control group was given no treatment and placed in a large animal laboratory without any interference.The white noise was played by computer and speaker until the specified time(2h,4h,6h),With the help of the HS5633 digital sound level meter,creating 60 d B,100 d B noise environment.Other environmental indicators are controlled within the scope of environmental standards GB14925-2010.Using self-matching experimental design,that is,before playing the noise(before the administration group was not gavage),0h after playing the noise and 1,3,4 days after playing the noise,respectively,with one-time venous blood collection needle with vacuum blood collection tube In the experimental group,the anterior vena cava blood of the most obvious site of beating chest thoracic cavity was used as the routine indicator of ion biochemistry and blood.Effects of Transportation on Biochemical and Ion Indexes of Blood Indices in Normal Pigs and Anti-stress Effects of Ginsenosides Twelve male common mini-pigs were divided into two groups(ginsenosid es group and control group),6 in each group,1h before the experiment,gi ving the administration group ginseng total saponin gavage,The control gr oup did not do any processing,using the usual means of transport in the urban areas of Guangzhou and the city highway transport(divided into 2h group,4h group,6h group),using their own paired experimental design,th at is,before transport(administration group did not before gavage),0h after transport and 1,3,4 days after transport,the same time,respectivel y,with one-time venous blood collection needle with vacuum blood collect ion two groups of experimental piglets chest anterior chamber beat most o bvious part of the anterior vena cava,used as ion biochemical and blood routine indicators.Result Effects of noise on ion biochemical indexes and blood routine indexes of ordinary miniature pigs and the anti-stress effect of ginsenosides 1.1Effect of 60 d B Noise Stress on Biochemical Indexes of Ion and General Blood Index of Common Mini Pigs and the Anti-stress Effect of Ginsenosides The noise of 60 d B has different effects on the blood routine indexes such as RBC,WBC,HGB,HCT,MCH,MCV,PLT and MCHC in ordinary miniature pigs,and the effect of ginseng total saponin can be reduced to a certain extent.The noise of 60 d B had different effects on the plasma biochemical parameters of calcium,sodium,potassium,chloride,ALT,AST,BUN,CREA,CHOL,TG,CK,LDH and GLU in ordinary miniature pigs.To some extent,its impact can be reduced.1.2 Effect of 100 d B Noise Stress on Biochemical Indexes of Ion and General Blood Index of Common Mini Pigs and the Anti-stress Effect of Ginsenosides The noise of 100 d B has different effects on the blood routine indexes such as RBC,WBC,HGB,HCT,MCH,MCV,PLT and MCHC in ordinary miniature pigs,and the influence of 100 d B noise on ordinary miniature pig blood routine is stronger than that of 60 d B noise.The total saponin to a certain extent can reduce its impact.The noise of 100 d B has different effects on plasma biochemical indexes of ordinary miniature pigs such as calcium ion,sodium ion,potassium ion,chloride ion,ALT,AST,BUN,CREA,CHOL,TG,CK,LDH and GLU,Level miniature pig ion biochemical index stronger than 60 d B noise,ginsenoside to a certain extent can reduce its impact.Effects of Transportation on Biochemical and Ion Indexes of Blood Indices in Normal Pigs and Anti-stress Effects of Ginsenosides 2h road transport stress will have different effects on the blood routine indexes such as RBC,WBC,HGB,HCT,MCH,MCV,PLT and MCHC in ordinary miniature pigs,and the total ginsenoside can reduce its influence to a certain extent.2h highway transport stress will have different effects on the plasma biochemical indexes such as calcium ion,sodium ion,potassium ion,chloride ion,ALT,AST,BUN,CREA,CHOL,TG,CK,LDH,Saponin to a certain extent can reduce its impact.4h road transport stress will have different effects on routine blood indexes such as RBC,WBC,HGB,HCT,MCH,MCV,PLT and MCHC in ordinary miniature pigs,and the total ginsenoside can reduce its influence to a certain extent.4h road transport stress ordinary mini-pig calcium,sodium,potassium,chloride,ALT,AST,BUN,CREA,CHOL,TG,CK,LDH,GLU plasma biochemical indicators have different effects,and ginseng total saponin To a certain extent,reduce its impact.6h road transport stress will have different effects on the blood routine indexes such as RBC,WBC,HGB,HCT,MCH,MCV,PLT and MCHC in ordinary miniature pigs,and the total ginsenoside can reduce its influence to a certain extent.6h road transport stress ordinary mini-pig calcium,sodium,potassium,chloride,ALT,AST,BUN,CREA,CHOL,TG,CK,LDH,GLU plasma biochemical indicators have different effects,and ginseng total saponin To a certain extent,reduce its impact.Conclusion Noise and transport to varying degrees,to a small pig digestion,hematopoiesis,immune function,liver and kidney function have some impact,some indicators can be restored after adaptive feeding;Ginseng total saponin can enhance the anti-stress ability of miniature pigs.
Keywords/Search Tags:noise, transportation, ordinary miniature pig, ginseng total saponin, anti-stress
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