Study On The Relationship Between TCM Constitution Types And Susceptibility Genes With Parkinson’s Disease

ObjectivesExplore the correlation between TCM constitution types and G2385R、R1628P、G2019S、S1647T、R1441C、A419V4 polymorhpism of LRRK2 genes with Parkinson’s disease,to determine whether the polymorphism of susceptibility genes in PD patients can influence or even determine the basis of the bias constitution type,The total homozygous mutation rate(3.27%)of the two loci was lower than that of heterozygous mutation rate(9.83%).The homozygous mutation rate was the same at G2385R(3.27%),and the R1628 P site was heterozygous mutation(6.56%).MethodsThe LRRK2 gene polymorphism of 61 patients with Parkinson’s disease with different constitutions was analyzed the snapshot gene detection technique.If the snapshot gene detects poor results,the PCR resequencing technique is used to further confirm the results.The correlation between different locus of LRRK2 gene was analyzed,and the differences between the patients with gene mutation and non-carriers in clinical symptoms,UPDRS scores were compared.Results1、There’re a total of 8 cases of 61 patients with PD in LRRK2 gene polymorphism,total mutation was 13.1%,including G2385 R,R1628P loci polymorphism each 4 cases,both locus mutation rate was 6.56%,the two locus mutation frequency comparison between the three groups,there was no statistically significant difference(P>0.05),more than 4 loci are found mutations.2、In the G2385 R site polymorphism detection results,there were 2 cases in the Yin deficiency quality group and all were heterozygous,and 1 case of phlegm wet quality group and the mild quality group was homozygous.Among them,the mutation rate of AA/GA genotype was 10%,5%,5%,and A allele mutationrate of 5%,respectively,in the Yin deficiency group,the phlegm wet quality group and the mild quality group.There was no statistically significant difference between the three groups of genotypes and alleles(P>0.05),and there was no statistically significant difference between the two groups(P>0.05).3、In the results of R1628 P polymorphisms,there were 3 cases and 1 case in the phlegm-dampness group,and the mild quality group,all heterozygous,and no mutation was found in the Yin deficiency group.The mutation rates of GC genotypes were 0,14.3% and 5%,respectively,in the Yin deficiency,the phlegm group,and the mild quality group,and the mutation rate of allele C was 0,7.1% and 2.5%.There was no statistically significant difference between the genotype and allele of the experimental group and the control group(P>0.05),and the difference was not statistically significant(P>0.05).4、In terms of clinical manifestations,there was no statistically significant difference in clinical symptoms,UPDRS scores and Hoehn-Yahr grading in patients with G2385 R or R1628 P site mutations(P>0.05).ConclusionsThe result of this study showed that LRRK2 gene G2385 R and R1628 P sites were polymorphisms,but there was no significant correlation between PD patients with different constitution types and LRRK2 genes.