Effets Of PRMT2 On Cisplatin Resistance In Breast Cancer MCF-7 Cells

Objective To investigate effect of knocking down of PRMT2 in MCF-7 breast cancer cell line on cisplatin resistance and explore the underlying mechanisms.Methods 1.Western blot was conducted to verify the stable infected MCF-7-PRMT2 knock down cell line.2.Cell viabilities of MCF-7-NC and MCF-7-PRMT2 were assessed by MTS after treatment of cisplatin.3.Flow cytometry was carried out to compare effect of different concentrations of cisplatin on apoptosis of MCF-7-NC and MCF-7-PRMT2 breast cancer cell lines.4.Western blot was used to detect the effect of different concentrations of cisplatin on PRMT2、P-Akt and P-ERK1/2 expression in MCF-7-NC and MCF-7-PRMT2 breast cancer cells.Results 1.The stable infected MCF-7-PRMT2 knock down cell line was successfully established.2.Compared to control group,MCF-7-sh PRMT2 cell line exhibited an increased cell viability after treatment of cisplatin.3.Decreased apoptosis was found in MCF-7-shPRMT2 cell line compared with control group.4.Western blots indicates that PRMT2 were down regulated in MCF-7-shPRMT2 cell line compared with control group.Meanwhile,P-akt was up regulated.However,expression of P-ERK1/2 showed no significant changes.When treated with different concentrations of cisplatin,P-akt was still up regulated when compared to control group.Conclusion Down regulation of PRMT2 may increase cisplatin resistance in MCF-7 breast cancer cell which is partly associated with up regulation of phosphorylation of Akt.