| 【Objective】To investigate the expression of SRPK2 in mi R-493 target gene and its corresponding normal tissues,and to provide a new prognostic index for tumor therapy.【Methods】PartⅠ:According to the target gene predicted by bioinformatics software,the difference in expression between tumor tissue and normal tissue was analyzed by Oncomine database.PartⅡ:The differential expression of SRPK2 protein in common tumors and normal tissues was verified by immunohistochemistry of tissue microarray.【Results】PartⅠ:Compared with PUM2,the high expression of SRPK2 in common tumors was more obvious,so we selected SRPK2 as the research object.In Oncomine,there were 25 studies involving SRPK2 expression in colorectal cancer tissues and normal tissues.Ten results showed that SRPK2 expression in colorectal cancer was significantly higher than that in normal tissues(P <0.01).Ten studies were recorded in the Oncomine liver database,and six studies have statistical differences.The results of survival analysis showed that the high expression of SRPK2 in hepatocellular carcinoma was associated with its low survival rate.The results of the analysis of gastric cancer database showed that the survival rate of high SRPK2 m RNA expression was significantly higher than that of low expression.PartⅡ:Immunohistochemical staining showed that the expression of SRPK2 protein in colorectal cancer,liver cancer and gastric cancer was higher than that in normal tissue,and the positive expression of SRPK2 protein was significantly higher in colorectal cancer tissues(P<0.01).The SRPK2 expression level positively correlated with Lymph node involvement.【Conclusions】1.SRPK2 was highly expressed in common tumors,the over-expression of SRPK2 in Colorectal Cancer was more obvious.2.Over-expression of SRPK2 in Colorectal Cancer was correlated with Lymph node involvement.SRPK2 play an important role in the progress of Colorectal Cancer. |
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