| Objective: To study the expression of leukemia inhibitory factor receptor(LIFR)in colorectal cancer(C RC),and to analyze the correlation with the clinicopathological features,and to explore the role and clinical significance of the expression of the leukemia inhibitory factor receptor in CRC.Methods: the tumor tissues of 39 patients who underwent colon cancer and(or)rectal cancer operation from October 2016 to April 2017 were collected and 15 cases of normal tissues adjacent to the cancer were taken as control.The distribution and protein expression level of LIFR in CRC and normal tissues were measured by immunohistochemical method,and the relationship between the expression level and the clinicopathological characteristics was analyzed.Western Blot was used to further detect the expression level of LIFR in CRC.PCR Real time quantitative Polymerase Chain Reaction(RT-PCR)was used to determine the difference of LIFR expression level in CRC tissues and normal tissues.The colon cancer cell strain(Lo Vo,SW620)of the logarithmic growth period was used to construct a colon cancer cell line(Lo Vo-LV-LIFR,SW620-LV-LIFR)and negative control group(Lo Vo-LV-NC,SW620-LV-NC),which had a stable up and up expression of LIFR(Lo Vo-LV-NC,SW620-LV-NC)through the transfection of the lentivirus.The cell scratch test and cell proliferation experiment were carried out to further detect the LIFR in CRC.Effect。Results: 1.The results of immunohistochemistry showed that LIFR was expressed in different degrees in CRC tissues,and the positive staining was mainly located in the cell membrane.In 39 cases of C RC,28 cases(71.8%)had low LIFR expression,while 14 cases(93.0%)showed normal expression in 15 normal colorectal tissues.2.The analysis of clinicopathological features found that the low expression of LIFR was associated with TNM staging and lymph node metastasis of CRC(P<0.05),but not related to the sex,age and tumor size of the patients with CRC(P>0.05).3.In Western Blot,14 cases(93.0%)of 15 normal tissues showed high expression of LIFR.In 39 cases of CRC,28 cases(71.8%)had low expression of LIFR.The results of 4.RT-PCR showed that the expression level of LIFR in CRC was significantly lower than that of normal tissues(0.0003±0.00469vs0.021±0.00531),and the difference was statistically significant(P<0.05).5.The scratch test showed that the 24 hour healing speed of the up regulation group was lower than that of the negative control group(Lo Vo-LV-LIFRvs Lo Vo-LV-NC,0.551±0.043vs1.000±0.044,P<0.01)after lentivirus transfection.In the SW620 cell line,LIFR expressed the 24 hour healing speed of the up-regulated group after the transfection of the lentivirus.The degree was also lower than that of the corresponding negative control group(SW620-LV-LIFRvs SW620-LV-NC,0.631±0.032vs1.000±0.036,P<0.01).6.The results of cell proliferation test showed that in the Lo Vo cell line,the absorbance of LIFR up regulation group after lentivirus transfection was lower than that of the control group(Lo Vo-LV-LIFRvs Lo Vo-LV-NC,0.581±0.025vs0.731±0.004,P<0.05),and the SW620 cell line was transfected by the lentivirus,and the LIFR expression of the up regulation group was sucked for fourth days.The luminosity was also lower than that of the control group(SW620-LV-LIFRvs SW620-LV-NC,0.564±0.013vs0.697±0.032,P<0.05).Conclusion: 1.The decreased expression of LIFR in colorectal cancer suggests that it is involved in the progression of colorectal cancer.2.The low expression of LIFR in colorectal cancer patients was associated with TNM stage and lymph node metastasis,but not related to sex,age and tumor size.3.LIFR maybe play an antitumor role in the process of CRC,and it can be an inhibitor in the proliferation and migration of tumor progression. |
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