| Objective : To detect the methylation status of pp ENK gene and TFPI-2 gene in pancreatic tissue,and to explore the feasibility and application of the methylation status detection of pp ENK gene and TFPI-2 gene in the pancreatic space occupying lesions obtained by EUS-FNA.Methods: The methylation status of CPG islands in the promoter region of pp ENK gene and TFPI-2 gene in 86 cases pancreatic cancer tissues and adjacent tissues were detected by bisulfite genomic sequencing PCR(BSP),and the clinica pathological characteristics between pancreatic cancer and adjacent tissues were analyzed by logistic.Results:In this study of 86 cases,the success rate of methylation was 91.9%(79/86).The detection rates of methylation of TFPI-2 gene and pp ENK gene genes were 95.9%(75/79)and 91.1%(72/79),respectively.There were significantly statistic differences between the tumor group and the normal control group(P<0.05),while there was no significant difference between the inflammatory group and the normal control group.The results of receiver operating characteristic curve showed that the sensitivity of TFPI-2gene and pp ENK gene methylation level in the diagnosis of pancreatic space occupying lesions were 83.6% and 74.6%,respectively,and the specificity was 81.8%and 86.4% respectively,diagnosis rate was 89.7% and 81.2% respectively.The diagnosis of pancreatic space occupying lesions sensitivity of the methylation level of TFPI-2 gene or pp ENK gene was 92.5%,the specificity was 79.8%,and the sensitivity of the TFPI-2 gene and pp ENK gene methylation level of diagnosis of pancreatic space occupying lesions was 74.6%,the specificity was 91.7%.Conclusions:TFPI-2 and pp ENK genes have high methylation level in pancreatic neoplasms and have good sensitivity and specificity.It is a useful diagnostic index for pancreatic cancer.Combined with EUS-FNA,it can effectively improve the preoperative diagnosis. |
PDF Full Text Request