The Influence Of Alginate 3D Cultured Fibroblast Cell On Hair Follicle Regeneration

Current researches about hair follicle regeneration mainly focus on reprogramming other types of cells to dermal papilla-like cells that participate in interacting with epidermis and forming hair follicles.As a type of cells present in skin tissues,fibroblast can be cultivated easily and stably.Furthermore,research has shown that in the presence of specific growth factors,fibroblast would obtain the capacity to promote the regeneration of hair follicle.In conventional 2D culture,fibroblast rarely express dermal papilla related-genes,and do not possess the capacity to promote hair follicle generation.As a biodegradable and biocompatible material,alginate has been widely applied.In the current study,alginate was employed to encapsulate fibroblast,allowing nutrition entry and metabolic waste exit thus creating a suitable 3D microenvironment for cell growth.Optimization of encapsulation parameters,such as capsule size,cell density,culturing period would help achieve high expression levels of dermal papilla-related genes in the encapsulated fibroblast cellsSpecifically,the following tasks would be fulfilled:1.Fabrication of alginate capsules with the diameter of 300 μm via electrostatic spraying and the optimization of fabrication parameters.2.Fabrication of the alginate capsules with different cell density and exploration of the relationship between encapsulation density of fibroblast cells plus cultivation period and dermal papilla-related gene expression.3.Construction of transplantation animal model and investigation of the feasibility of 3D-cultivated dermal papilla-like cells in regenerating hair foliccle.