Chemical Constituents From Cremastra Appendiculata

Crematoria appendiculata(D.Don)Makino was one of the source of traditional Chinese medicine“shancigu”.It was cool in nature,light pungent and sweet in taste.It has actions of removing heat and counteracting toxicity,dissipating phlegm and resolving masses.It can be applied in the treatment of carbuncle and furunculosis,scrofulous sputum core,snake bitesThe compounds of Crematoria appendiculata were isolated by repeated column chromatography with silica gel,Sephadex LH-20 and ODS-HPLC.The struct-ures were elucidated by analysis of spectroscopic data.Twenty-five compounds were isolated and their structures were identified as 2’,7’-bihydroxy-4,4’,-bimetho xy-1,1’-biphenanthrene-2,7-di-O-β-D-glucoside(1),2’-hydroxy-4,4’,7’-trimethoxy-1,1’-biphenanthrene-2,7-di-O-β-D-glucoside(2),1-(4-Hydroxybenzyl)-4-methoxy-2,7-dihyd-roxyphenanthrene-8-O-β-D-glucoside(3),3′,5-dihydroxy-2-(p-hydroxybenzyl)-3-meth-oxybibenzyl(4),3’,5-dihydroxy-3-methoxy-2,4-di(p-hydroxybenzyl)bibenzy(5),2,7-di-hydroxy-1-p-hydroxybenzyl-4-methoxy-9,10-dihydrophenanthrene(6),1-(p-hydroxybe nzyl)-2,7-dihydroxy-4-methoxyphenanthrene(7),3,3′-dihydroxy-5-methoxy-2,4-di(phydroxybenzyl)bibenzyl(8),4,7-dihydroxy-1-p-hydroxybenzyl-2-methoxy-9,10-dihydrophenanthrene(9),5,4′-bihydroxy-bibenzyl-3-O-β-D-glucoside(10),3′-β-D-glucopyranosyloxy-4,5′-dihydroxy-3-methoxy-l,2-diphenylethane(11),cirrhopetalanthin(12),lariciresinol(13),Blespirol(14),lignan glycosides(15),4-O-(6′-O-glucosyl-p-coumaroy l)-4-hydroxybenzylalcohol(16),β-D-Glucopyranose,6-[3-(4-hydroxyphenyl)-2-pro-pen oate](17),2,7-dihydroxy-4-methoxy-9,10-dihydrophenanthrene(18),3,5,3′-trihydroxybibenzyl(19),7-hydroxy-2-4-dimethoxy-phenanthrene(20),batansin III(21),β-D-Glucopyranoside-4-(2-hydroxyethyl)phenyl(22),β-daucosterin(23),3-hydroxybenz-cicacid(24),4-hydroxy-2-methoxypheny-1-O-β-D-glucopyranoside(25)Compounds1-3 were new Compounds;Compounds 5-6,8-11,13-17,21-22,24-25 were is-olated from Cremastra for the first time.The isolated compounds 1,4,6,7,12,18,19,20,21 were screened by cytotoxity experients.Compounds 1,12,20 showed moderate cytotoxic activity to inhibit BT474 and SKBR3 cells.This study provide a basis for further development of Crematoria appendiculata...