DOT1L And PAF1 Synergistically Regulate 3T3-L1 Differentiation

Obesity is a serious health problem around the worldwide now,it’s necessary to understand the mechanism of adipocyte differentiation to develop treatments for obesity.It’s well know that DOT1L plays very important role in the development of MLL-rearranged leukemia,and some drugs has been developed to treat such leukemia by targeting DOT1L.However,researchers also noticed that DOT1L play important roles in cell differentiation,yet the mechanism is not well understood for now.Most of the researchers speculate that DOT1L regulates many important differentiation-relative genes expression through epigenetic modifications on H3K79 to influence cell differentiation.In this article we used 3T3-L1 cells which is Classic cell model for adipocyte differentiation research to research that:(1)Does DOT1L play important roles in adipogenesis?(2)If it dose play roles in adipogenesis,what’s the regulation mechanism?Our experimental results show that DOT1L is necessary for adipogenesis.However our regulation mechanism research data shows DOT1L may not influence 3T3-L1 differentiation though it’s role in H3K79 modifications which is very different from most researchers’ speculation.These findings confirm the important role of DOT1L in adipogenesis and provide new ideas for developing drugs against obesity and relative diseases.And also provide a new perspective about the mechanism of DOT1L regulate cell differentiation.