Chemical Constituents From The Antiosteoporosis Active Fraction Of Sambucus Willamsii Hance

Sambucus williamsii Hance is one species of Sambucus genera in Caprifoliaceae.It has the potence of expelling wind-dampness,rejoining fracture and restoring soft tissue,invigorating blood circulation and stopping pains.Our previous study found that the 60%ethanol crude extract of the stems of Sambucus williamsii Hance was eluted by D101 macro adsorptive resin column with a gradient of ethanol/water(0,30%,50%and 95%),and JGM(the fractions of 50%and 95%)was considered as the antiosteoporosis active fraction by increasing the proliferation activities of UMR106 cells and preventing ovariectomy-induced bone loss in rats.To explore the potential bioactive constituents,we have a systematic chemical investigation on the antiosteoporosis active fraction of Sambucus willamsii Hance.29 compounds were isolated from JGM through various chromatographic techniques,including 15 lignans,8 phenolic acids,3 triterpenoids and 3 other kinds of compounds.The structures were elucidated on the basis of theirs physicochemical properties and spectroscopic characters as(7R,8S,7’R,8’S)-syringaresinol(1);(7R,8S,7’R,8’S)-medioresinol(2);(7S,8R,7’S,8’R)-pinoresinol(3);(7S,8S,7’R,8’S)-lirioresinol A(4);(7S,8R,7’S,8’R)-1-hydroxypinoresinol(5);(7R,8S)-ficusal(6);(7R,8S)-5-methoxybalanophonin(7);(7R,8S)-balanophonin(8);(7R,8S)-dihydrodehydrodiconiferyl alcohol(9);(7R,8S)-5-methoxy-trans-dihydrodehydrodiconiferyl alcohol(10);(7R,8S)-1-(4-hydroxy-3-methoxyphenyl)-2-[-4-(3-hydroxypropanyl)-2-methoxyphenoxy]-1,3-propanediol(11);(7S,8S)-1-(4-hydroxy-3-methoxyphenyl)-2-[-4-(3－hydroxypropanyl)-2-methoxyphenoxy]-1,3-propanediol(12);(7S,8S)-guaiacylglycerol-β-O-4’-conifery ether(13);(7S,8R,7’S)-cycloolivil(14);(7S,8R,8’S)-isolariciresinol(15);vanillin(16);p-hydroxybenzaldehyde(17);octadecyl-(E)-ferulate(18);eicosyl-(E)-ferulate(19);docosyl-(E)-ferulate(20);2-propenoic acid,3-(4-hydroxy-3-methoxyphenyl)-(9Z)-9-octadecenyl ester,(2E)-(9C1)(21);octadecyl-(E)-caffeate(22);eicosyl-(E)-caffeate(23);23-hydroxy-3-oxo-28-nor-12,16-dien oleanane;Sambucusan A(24*);oleanolic acid(25);3-oxooleanolic acid(26);octacosanol(27);hexadecanoic acid(28);βsitosterol(29).Among them,1 compound(24)was a new compound and 11 compounds(5,7,8,10,17-23)were isolated from the Sambucus genus for the first time.The proliferation effects of 28 isolated compounds on osteoblast-like UMR106 cells were determined.The results showed that 18 compounds(3～17,21,24,25)significantly increased UMR106 cell proliferation.Furthermore,the study has also established the HPLC specific chromatogram of the antiosteoporosis active fraction from Sambucus williamsii Hance,and identified the main peaks(occupied more than 60%total peak areas)by all isolated compounds.Our present investigation provided the scientific evidence for research and developement of Sambucus williamsii Hance and its related preparations on osteoporosis in the future.