Kdney Invigorate The Party Against Cardiolipin Antibody Positive Model Mice Serum TLR4,the Influence Of The Nf-kappa B Experimental Research

Objective:Observe kidney invigorate the party against cardiolipin antibody positive lead to recurrent miscarriage TLR4,the influence of the nf-kappa B.Through experimental research,the animal model of mice serum anti cardiolipin antibody,resistance to beta 2 gpi antibodies,TLR4,the nf-kappa B level,through the kidney invigorate the circulation of the high,medium and low dose compared with aspirin,preliminarily TLR4/the nf-kappa B pathway in anti cardiolipin antibody positive result in the transmission mechanism of recurrent miscarriage,investigate whether the kidney invigorate the party can reduce the anti cardiolipin antibody,resistance to beta 2 gpi antibody levels,regulate the body’s immune function,for kidney invigorate the circulation in the disease provide experimental basis for clinical applicationsMethods:Establishing anti cardiolipin antibody,resistance to beta 2 glycoprotein 1 antibody positive animal models in mice,50 BALB/c mice were randomly divided into 6 groups,namely A:kidney invigorate the circulation of the high dose group.B:kidney invigorate the circulation of the dose groups,c:kidney invigorate the circulation of the low dose group,D:western medicine group(aspirin),E:model group,F:blank group.Day 1,in addition to the blank group,the others all intraperitoneal injection after add freund’s complete adjuvant,such as volume of epoetin beta 2 gpi solution 50 mu L/only.Day 8,injection volume injection to join such incomplete freund’s adjuvant deployment of epoetin beta 2 gpi solution after 50 mu L/only,strengthen the immune after 10 days,male and female mice in a 2:1 ratio and cage,to find a large number of sperm or vaginal suppository day 1 for pregnancy,with corresponding medicine lavage,15 days in a row.15 days after the mice to death by ELISA in serum was detected in two antibodies(ACA,AntiB2GPI)and two kinds of cytokines(TLR4,the nf-kappa B)level.Result:Mice kidney invigorate the party can reduce serum ACA,resistance to beta 2 gp Ⅰ antibody,TLR4,the nf-kappa B level,and kidney invigorate the circulation of the dose effect is best,curative effect is comparable to aspirin.Conclusion:1.TLR4/nf-kappa B signaling pathways and ACA(+)RSA;2.kidney and activating blood can by adjusting the TLR4/nf-kappa B signaling pathways related the expression of cytokines,inhibit the pathway excessive expression,and ultimately achieve kidney invigorate the circulation,remove blood stasis fetus.