The Mechanism Of Oral Absorption After Coadministraion Of Uncaria And Gastrodia

Uncaria and Gastrodia are commonly used in the treatment of hypertension.Uncaria main active ingredients are Rhynchophylline and Isorhynchophylline.Gastrodia active ingredient is Gastrodin.In this paper,the Caco-2 cell model and Pharmacokinetic model were used to study.The main active ingredients of Uncaria&Gastrodia of a long intestinal absorption mechanism,in particular the different compatibility of Uncaria&Gastrodia drugs in vivo absorption of drugs.In this paper,by examining the different extraction processes to extracted active ingredients from Uncaria and Gastrodia.Gastrodin(GAS),Rhynchophylline(RHY)and Isorhynchophylline(ISO)were established by HPLC determination,and validation of the methodology.The results meet the method requirements,the three components of the cell samples used for determination.The research based on compatibility proportion,clinical study,optimum extraction and others references.The compatibility proportion in 12:9、10:10、15:10、15:6 and 5:1 of Uncaria and Gastrodia were dissussed for issue.GAS,RHY and ISO were established by the HPLC determination in vitro Caco-2 cell culture model.Using in vitro Caco-2 cell culture model of the active ingredients from Uncaria and Gastrodia made the study of absorption mechanisms comprehensively.Gastrodin has no toxic effects.RHY and ISO has a certain toxicity.The 12:9 of Uncaria and Gastrodia has the highest safely range.RHY,ISO,and GAS were researched in the cellular transport,while P-gp,MRP,SGLT1 and GLUT2 transporter proteins were impacted on the absorption process to observe and study the absorption mechanism.ISO and GAS was mainly passived transport in Caco-2 cells.They was not effected by SGLT1,GLUT2,P-gp and MRP in the small intestine transport.RHY may cross the Caco-2 cells by active transport.P-gp and MRP were influenced on the cellular transport of RHY.We studied the vivo pharmacokinetic behavior of three active ingredients(RHY,ISO,and GAS)in healthy rats.And determined the plasma concentration of active ingredients after administration at different times.Compared pharmacokinetic process changes with separate administration and compatibility administration,to explore the compatibility of the mechanism of Uncaria and Gastrodia and scientific rationality.The pharmacokinetic parameters of the statistical analysis results showed that the 12:9 of Uncaria and Gastrodia can significantly increase in vivo plasma concentration of three-active ingredients.The passage studies on Uncaria and Gastrodia main active ingredients of the mechanism of intestinal absorption and major effect of the compatibility of active ingredients.All the dates of the studies make the base of the further research and second study.