1.A Case Report Of Primary Cutaneous Mucinous Carcinoma And Review Of The Literature 2.Sensitivity Of Caucasian And African-American Primary Melanocytes To Induction Of Autophagy Under Amino Acid Starvation Or Serum Starvation In Vitro

Background:Primary cutaneous mucinous carcinoma is a rare skin tumor frequently occurred in the eyelid of man over 50.It is difficult to differentiate this carcinoma histologically from cutaneous carcinoma metastases of breast or colonal tumors.Therefore,a thorough examine of the patient’s overall condition is of great value to rule out the metastases from breast or gastrointestinal tumors on the basis of histopathologic diagnosis.Case report:In 2011 a 47-year old man came to our hospital with a painless nodule in the left palpebra inferior.Clinical examine showed that there was a 0.5*0.6cm,movable nodule.No palpable lymph node was examined.Histology of the nodule showed mucinous carcinoma in the left eye lid.No tumor was found on patient’s chest X-ray and abdominal color Doppler ultrasound.According to the clinical information above,the diagnosis of primary cutaneous mucinous carcinoma was reached.The patient refused to have the surgery in our hospital and had the tumor removed in Beijing 1 year ago.We followed the patient after his surgery.No recurrency was found in the original location of the tumor.There was no aberrant sign in the patient’s chest X-ray and abdominal color Doppler ultrasound.Conclusion:Primary cutaneous mucinous carcinoma should be considered when slow-growing tumor occurred in the periocular region of the older male patients.There is no significant clinical sign in most cases of this eccrine-original tumor.Therefore,the diagnosis is delayed in many clinical cases.Furthermore,it is important to differentiate this tumor from mucinous carcinoma metastasis since there is no significant difference between these two tumors,which could help the patient have the tumor removed in time.Objective:To investigate and compare different level of autophagy in melanocytes derived from Caucasian and African-American neonatal foreskin induced by two nutrient starvations.Methods:Human melanocytes were isolated from foreskin of Caucasian and African-American newborns.Primary melanocytes were treated with complete medium,amino acid starvation and serum starvation separately in different time points.The level of autophagy in two types of melanocytes under different treatments among different time points were observed.Items to measure the level of autophagy under different nutrient conditions include:percentage of melanocytes undergoing autophagy in different time points by immunofluorescent staining;level of LC3B protein and turnover of LC3b I to LC3B II of melanocytes via western blot;function of autophagy to melanogenesis by measuring melanocyte dendritic length and intracellular melanin.Results:There is a basic autophagy level in both Caucasian and African-American melanocytes.Under amino acid starvation and serum starvation,human melanocytes undergoing autophagy are both time-dependent according to data on cell morphology and protein level.Under same treatment,African-American melanocytes reach the autophagic influx peak at an earlier time phase comparing to that in Caucasian melanocytes.Conclusion:1.Both Caucasian and African-American melanocytes have the intrinsic level of autophagy.2.According to the data on cell morphology and autophagic protein level of both Caucasian and African-American melanocytes,the level of autophagy are both elevated and time-dependent.3.Comparing melanocytes under same condition,African-American melanocytes response to intense starvation faster than Caucasian melanocytes.However,the former resists to mild starvation longer.4.Both melanocytes under different starvations have aberrant cell morphology and melanogenesis imbalance.