Study On The Real-time Cell Electronic Analysis Technology For Quality Evaluation Of Traditional Chinese Medicines

At present,the development of traditional Chinese medicines has been hampered to a large extent by the lack of compatible analytical approaches.In this research,a novel cell-based analytic approach,the real-time cell electronic analysis technology,would be used to authentication and quality control of nature medicines.This technology is based on continuous monitoring of cellular impedance in real-time,which will produce specific time/dose-dependent cell response profiles(TCRPs)upon treatment with the traditional Chinese medicines.The traditional Chinese medicinal Flos Carthami was discussed as a methodological example,and we explored the potential use of the real-time cell electronic analysis technology in analysis of traditional Chinese medicines.By analyzing the similarity of the TCRPs which produced by the Flos Carthami,we found that the 5 samples of Flos Carthami(come from 5 different regions)produced the highly similar TCRPs.Additionally,we evaluated the quality of Flos Carthami by HPLC and the TCRPs analysis-based approach respectively.The results evaluated by the two approaches have consistency.On the basis of these results,we suggested that the same kind of nature medicines can produce similar TCRPs.By analyzing the TCRPs,we could evaluate the bioactivity of nature medicines and further evaluate their quality.Also the real-time cell electronic analysis technology is a physiologically relevant approach for authentication and quality control of traditional Chinese medicines.