Pharmacology Studies On Zao Ling Anshen Compound

With the increase of social progress and working pressure,insomnia has become a common disease of modern society,and also one of the main symptoms of sub-health state.Incidence were more than 40%of chronic in adults and elderly patients.In daily life,medicated died get much favorite for the features of safety-low cost.Therefore,medicated diet as the background,having the theoretical and practical significance to develop prescription that select from sedative-medicated diet.By uniform and orthogonal design,Pentobarbital sodium threshold dose of sleep percentage and pentobarbital pentobarbital hypnosis time as the pharmacodynamic index,using stepwise multiple regression to variable selection.Determined the pharmaceutical composition and the optimal dose of compound by uniform design,setting Poria and Semen be a factor as optimal dose from Suanzaorentang,plus Lilium Longan Oyster Mulberry and walnut.But because of the interfere with not significant drug,as well as the limitations of dose range selection.By orthogonal design experiment,split the factor 1-Poria and Oysters by 6:5,setting 0 and a larger dose as two levels,to determine the contribution to the sleep indicators of each drug,and clearly the contribution to subthreshold dose of sodium pentobarbital sleep percentage and threshold dose of pentobarbital sodium in mice induced by sleep time of the compound different component.Removed the not significant effect sedative drug,reference single medicine clinical dose,pharmaceutical dose,and the civil diet used,appropriate to increase the maximum dose of the drug within the usual dose ranges,re-use of uniform design optimization for the Anshen compound.In the multi-group theory group by SPSS stepwise regression analysis obtained,Screening the higher OD theoretical group for validation experiments,determined the Zao Ling Anshen compound supplemented Longan,Walnuts with Semen,Poria and Oysters as main component.Divided mice into different indicators of A,B groups according two efficacy evaluation in drug onset time experiment.Interval experiment to different time points for A,B groups,to reduce error and the impact of drugs on mice.With the days of administration increase,showed hypnotic effect that 7 days after intragastric administration,Zao Ling Anshen compound could significantly prolonged pentobarbital sodium-induced sleeping time(P<0.01),improve subthreshold dose of sodium pentobarbital sleep percentage(P<0.01).Pharmacodynamics of Zao Ling Anshen compound from high,medium and low dose group by the general behavior of mice,pentobarbital sleep latency,subthreshold dose of sodium pentobarbital hypnosis experiment,threshold dose of pentobarbital sodium in mice induced by sleep time effects.The results showed:Zao Ling Anshen compound high,medium and low-dose mice had no significant direct sleep action;And the high-dose group,diazepam group can significantly increase the percentage of mice to sleep.Establish a method for the determination of the date Ling sedative compound total saponins in Semen UV spectrophotometry.Compound by orthogonal experiment to optimize the extraction process:plus 10 times the water,extracted 2 times 2 hours.Optimization of a prescription sedative effect have food and medicine used along.In order to further the development of sedative effect of new products laid the foundation.