The Correlation Between Blood Hcy Level And Bone Density In Elderly Women

Objective:To study the relationship between serum homocysteine（Hcy）and bone density（bone mineral density,BMD）in elderly women,to know the effect of HCY on bone mineral density in elderly women.Methods:1.we collected 201 cases elderly women aged above 60 years from our hospital in September2016 to Dec 2017.Using the American GE company’s dual energy X-ray detector to determine the participants（L1^L4）of the lumbar spine,femoral neck（FN）and total hip（TH）of bone mineral density.According to the results of dual energy X-ray BMD examination,subjects were divided into normal bone mass group（n=42）,osteopenia group（n=98）and osteoporosis group（n=61）.2.The age,menopausal age,menopause duration,height and weight were recorded ang measured,then calculated the bady mass index（BMI）.The level of blood calcium（Ca）,phosphorus（P）,alkaline phosphatase（ALP）,triglycerides（TG）,low density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C）and albumin were detected by Roche Diagnosis Ltd production of fully automatic biochemical analyzer.Hcy was measured using enzyme linked immunosorbent assay（ELISA）.3.The data was analysed by SPSS20.0 including one-way ANOVA,Pearson correlation analysis,multi-factor Logistic Regression Method.P<0.05 for the difference was statistically significant.Results:1.Comparison of data between the three groups:42 cases of bone mass normal group,the averageageof67.18±3.31,osteopeniagroup98cases,theaverageageof67.84±3.28,osteoporosis group 61 cases,the average age of 67.98±3.36.There was no statistical difference in three group’s age（P>0.05）.2.Comparison of the menstrual history of the three groups:the premenopausal age of the OP group and the osteopenia group was earlier than that in the normal group（46.62±2.84、46.89±3.94、49.48±2.46,P<0.01）.The menopause duration of OP group and osteopenia group was longerr than the normal group（20.79±3.24、20.14±2.53、18.71±2.26,P<0.01）.3.The gestation and chikdbirth history of the three groups:the OP and osteopenia group’s gestation（4.64±1.59、4.57±1.36、3.76±1.20）and chikdbirth（4.23±1.45、4.03±1.24、3.40±0.96）was more than normal group,the difference was statistically significant（P<0.01）.4.The BMI of the three groups:BMI in the OP group and the osteopenia group was laeger than that in the normal group（19.46±1.99、20.96±2.46、22.26±2.09,P<0.01）.5.The Hcy and general biochemical indicators of the three groups:Albumin,HDL-C,Ca,P and ALP were not statistically significant（P>0.05）.The level of HCY in OP group and osteopenia group was higher than that in the normal group（10.78±3.13、17.44±3.06、26.25±4.37,P<0.01）.Compared with the normal group,the level of LDL-C in the OP group and the osteopenia group is increased（2.54±0.70、3.22±0.90、3.22±1.03,P<0.01）.The TG level of the normal group was higher than that of the osteopenia group and the OP group（1.53±0.60、1.41±0.49、1.27±0.42）,but there was no statistically significant difference between the normal group and the osteopenia group.6.Pearson correlation analysis showed that there was no correlation between the age of menopause,albumin,HDL-C,Ca,P,ALP and L1-4,femoral neck,and total hip bone density.Age（r=0.222,r=0.268,r=0.279,P<0.05）,age of menopause（r=0.431,r=0.309,r=0.411,P<0.01）were positively correlated with the bone density of L1-4,femoral neck and total hip.The bone density of L1-4 was positively correlated with TG（r=0.139,P<0.05）and BMI（r=0.311,P<0.01）.The bone density and Hcy（r=-0.531,r=-0.351,r=-0.324,P<0.01）,and LDL-C（r=-0.181,r=-0.212,r=-0.159,r=-0.159,P<0.05）were negatively correlated.At the same time,the bone density and the pregnancy rate（r=-0.224,P<0.01）and the childbirth（r=-0.207,P<0.01）of L1 and 4 were negatively correlated.7.Logistic regression analysis showed that:with bone mineral density decreased as the dependent variable,in menopause age(X₁),menopause duration（X₂）,progesterone（X₃）,production time（X₄）and BMI（X₅）,HCY（X₆）,TG（X₇）,LDL-C（X₈）as the independent variables,multivariate linear regression analysis,the results show that the elderly women menopause age,menopause duration,BMI,gestational,childbirth,Hcy and LDL-C into regression equation.Regression equation:y=-2.714-0.558X₁+0.564X₂-2.299X₃-2.037X₄-1.95X₅+1.312 X₆+1.25 X₈Conclusion:1.The level of Hcy is significantly negatively correlated with bone density,and the level of Hcy can be used as a reference for evaluating the loss of bone mass in elderly women.2.Risk factors affecting the bone density of elderly women include:menopause duration（OR=1.758）,gestational（OR=9.962）,childbirth（OR=0.13）,HCY（OR=3.715）,and LDL-C（OR=3.491）.Protective factors affecting the bone density of elderly women include:menopause age（OR=0.572）and BMI（OR=0.823）;3.The level of LDL-C is significantly negatively correlated with bone density.