Expression Of LIMK2 In Ovarian Cancer And Its Effect On Cell Biological Behavior

Objective: The aim of this study was to investigate the expression of LIM kinase 2(LIMK2)in high grade serous ovarian cancer and normal ovarian tissue and to observe the effect of using si RNA technology to inhibit the expression of LIMK2 on cell biological behaviors in CAOV3 and OVCAR-3 ovarian cancer cell lines.And then to clarify the role of LIMK2 in the development and progression of high grade serous ovarian cancer.Methods: The expression of LIMK2 protein was detected by immunohistochemistry and western blot,the expression of LIMK2 m RNA was detected by real-time PCR(RT-PCR)in 41 cases of high grade serous ovarian cancer tissues and 30 cases of normal ovarian tissues,analyzed the relationship between its expression and clinicopathologic parameters.In the two cell lines,the observation group LIMK2-si RNA,the negative control group N-si RNA and the blank control group were constructed respectively.RT-PCR method was used to detect transfection efficiency.Cell scratch test was used to detect cell migration ability.MTT assay was used to detect cell proliferation.Transwell migration assay was used to detect cell invasiveness and flow cytometry was used to detect cell apoptosis.Results: The positive rate of LIMK2 in high grade serous ovarian cancer was significantly higher than that in normal ovarian tissue,LIMK2 expression was significantly correlation with FIGO stage and the level of CA125(P<0.05).However,the expression of LIMK2 had no significant difference with the age of ovarian cancer patients,lymph node metastasis,tumor size and ascites(P>0.05).Compared with the negative control group Nsi RNA and blank control group,the migration and invasion ability of LIMK2-si RNA cells in the observation group was significantly decreased(P<0.05),and the proliferation and apoptosis ability of the observation group was not significantly different from that of the control group and the blank control group(P>0.05).Conclusion: LIMK2 can promote the migration and invasion of ovarian cancer cells,and may be a new target for the treatment of ovarian cancer.