| Objective:In this study,we use senna to establisn rats with hyperfunctionally intestinal motility as a research object.BDNF-TrkB pathway which effects intestinal motility is regarded as target.We will discuss the effect and mechanism of electro-acupuncture back-shu and front-mu points for treating hyperfunctionaly intestinal motility rats.Methods:Different concentrations of senna Decoction were used to establish rats with hyperfunctionally intestinal motility and observed the stability of the rat model.A total of 30 SPF adult male rats were randomly divided into 5 groups:blank group,model group,back-Shu point group,front-Mu point group and Yu-Mu point group,6 in each group.The rats in the blank group were fed with normal saline every day.The other 4 groups used 0.3g/ml Senna Decoction to establish hyperfunctionally intestinal motility mode with lOml/Kg.After the gavage,the blank group and the model group were bound.With the same bounded method was used,back-Shu point group,front-Mu point group and Yu-Mu point group were treaded by corresponding electro-acupuncture methods.The needle was inserted into the Tianshu point and/or the large intestine Shu point.The depth of needling was about 5mm,and then needle handles were connected with the acupuncture treatment instrument.The electroacupuncture parameters were the density wave(sparse wave 2 Hz,dense wave 10 Hz),and the muscle light was light in rats,and the needle is left about 15 minutes.After tenth days of intervention,the time of first particle black stool in each group was observed.After 8 hours of fasting,animals were killed and rat intestinal tissue was taken.The difference in the expression of BDNF protein in intestinal tissue was detected by immunohistochemistry.The content of TrkB and p-TrkB in intestinal tract was detected by Western blot,and the effect of Electroacupuncture on the activation of TrkB receptor was investigated.Result:1.Using the high concentration(0.3g/ml)Senna Decoction to establish rats with hyperfunctionally intestinal motility is the best method.2.After electro-acupuncture treatment,the time of first grain black stool in each electroacupuncture group was significantly higher than that in the model grorup.The curative effect of back-Shu point group was the best,while that of Yu-Shu point egroup was the second,and the front-Mu point group was the lightest.3.Comparing the content of BDNF protein in the intestinal tissue with the electroacupuncture groups:compared with the model group,the content of BDNF protein in the intestinal tissue of front-Yu point group,Yu-Mu point group and back-Shu point group decreased.And the decrease of BDNF protein in front-Yu point group was the most obvious,and the decrease of BDNF protein in Yu-Mu point group and back-Shu point group was the lightest.4.Comparison of the content of Trk B and p-TrkB protein in the intestinal tissue between the groups:compared with the model group,the TrkB protein content of front-Yu point group and the Shu-Mu point group was significantly increased,and the p-TrkB protein contdent decreased significantly(P<0.01).Compared with model group,Content of TrkB protein increased significantly,while the content of p-TrkB protein decreased significantly,and the differences were statistically significant(P<0.001).Conclusion:1、Senna water decoction can accelerate intestinal motility establishment of rat model of intestinal motility in rats,but the effect of Senna on intestinal motility is not lasting,which is unable to maintain the stability of the model after stopping using drug.2、To speed up the action target of senna intestinal motility in rats may be BDNF-TrkB pathway in intestinal tissues.BDNF protein has the effect of accelerating intestinal motility,and its mechanism is related to the formation of p-TrkB protein after the combination of BDNF protein and its high affinity receptor TrkB.3.Electroacupuncture at back-Shu point and Yu-Mu points can reduce the p-TrkB and accelerate the intestinal motility by reducing the binding of BDNF protein and its high affinity receptor TrkB,and regulating the intestinal motility of the rats with intestinal hyperdynamic. |
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