Study On Chemical Constituents And Potential Anti-depressive Activity Of The Seeds Of Caesalpinia Sappan L.

Caesalpinia sappan L.is a species in Caesalpinia（Leguminosae）.The dried heartwood of C.sappan is a traditional medicine,which has been used for years in China.It can promote blood circulation,antibacterial and anti-inflammatory function.This thesis focuses on the chemical investigation and potential anti-depressive activities of cassane diterpenoids isolated from the seeds of C.sappan.The compounds were isolated and purified by repeatedly chromatography with silica gel,Sephadex LH-20,ODS（C-8 and C-18）,and MCI.The structures were identified by IR,NMR and MS methods.23 compounds were isolated among which18 compounds were identified.They are 9,10-O-isopmpyllidene-（12 Z）-en-octadecaoic acetate（CS-01）,β-sitosterol（CS-02）,24-ethyl-5,22-cholestadien-3-beta-ol（CS-03）,phanginin A（CS-04）,phanginin L（CS-05）,phanginin C（CS-06）,18-acetoxycassa-12,15-diene-3-one（CS-07）,apigenin（CS-08）,vanillic acid（CS-09）,biochanin A（CS-10）,daucosterol（CS-11）,β-amyrin（CS-12）,spathulenol（CS-13）,cycloeucalenol（CS-14）,β-sitosterol palmitate（CS-15）,cycloart-23-ene-3β,25-diol（CS-16）,quercetin（CS-17）,phanginin H（CS-18）,caesalppans G（CS-19）.Among them,CS-01 is a new coumpound,CS-06,CS-08,CS-12,CS-13 and CS-14 were isolated from this plant for the first time.Neuroprotective effect of cassane diterpoids was studied in corticosterone-induced PC-12 cell neurotoxicity.Cell viability was assessed by MTT assay.The released amount of lactate dehydrogenase（LDH）and[Ca²⁺]_i concentration were measured.The results indicated that the compounds extracted from the seeds of C.sappan could bring neuroprotective effect,proving a possible action pathway by decreasing LDH release and[Ca²⁺]_i concentration.