Effect Of HMGB1 On MyD88 And NF-κ B In Syndrome Ulcerative Colitis Rats By Shaoyaotang

Objective: 1.The study,through literature review in recent years,explored the pathogenesis and treatment of ulcerative colitis by traditional Chinese medicine,screened the Chinese medicine and then conducted experimental intervention.The influence of the high mobility group protein B1(high mobility group protein B1,HMGB1),myeloid differentiation factor MyD88 and nuclear factor kappa B(NF-kappa B)in the colonic tissue of the UC rat model of damp heat intramedullary model was observed.And the effect of on HMGB1-TLR signaling pathway.2.With the role of the representatives of qingrelishi side to observe Shaoyaotang,damp heat ulcerative colitis(ulcerative colitis,UC)of high mobility group protein B1 in colon tissue in a rat model(high mobility group protein B1,HMGB1),the adaptor protein MyD88 and nuclear transcription factor kappa B(NF-K B)the effect,explore the relationship between HMGB1-TLR pathway and damp heat type UC and Shaoyao Decoction on HMGB1-TLR pathway.Methods: The model of combination of disease and syndrome rehabilitation,which is with high fat and high glucose diet and spicy immune complex method(2,4,6-+ TNBS three trinitrobenzene sulfonic acid)model combined with ethanol composite method,Wistar female rats 60 rats each,divided into blank group,model group,Shaoyaotang high,medium and low dose group,sulfasalazine pyridine sulfonyl group,high,medium and low Shaoyaotang dose administered,sulfasalazine group received sulfasalazine,grinding into powder and configured medicine volume of liquid gavage,blank group and model group were given equal volume of saline,continuous 21 D.The colonic tissue,using real-time fluorescence quantitative PCR method(Real-time PCR)assay and Western blot to detect protein expression(Western blot),Su eosin(HE)staining standard pathological sections.Result: 1.Activities and states After modeling,all aspects of each activity and mental status were poor,and there are different degrees of diarrhea,and varying degrees of dark gray;after Shaoyaotang and sulfasalazine intervention,mental state of rats and the activity of each treatment were significantly improved,compared with model group,high Shaoyaotang dose group was the most obvious,the DAI score was statistically significant(P<0.05);2.Colonic histopathology The model with different degrees of defect surface to the muscularis mucosa group,Shaoyaotang and sulfasalazine intervention of neonatal visible glands,each mucous layer were repaired in high dose group was most obvious;3.Expression of mRNA and protein in colon tissue Compared with the control group,HMGB1 model group,MyD88,expression of NFkappa B protein and mRNA increased significantly(P<0.05);compared with the model group,Shaoyaotang and sulfasalazine group,group HMGB1,MyD88,NFkappa B protein and mRNA expression were decreased in different degree,Shaoyaotang high dose group and Salicylazosulfa Pyridin group(P<0.05).Conclusion: The occurrence of 1.UC is associated with the HMGB1-TLR pathway,which may be one of the mechanisms of the pathogenesis of UC.2.Paeoniae decoction can inhibit HMGB1 gene in damp heat UC rats,thereby regulating HMGB1-TLR pathway,inhibiting MyD88 and NF-kappa B gene expression,and decreasing the inflammatory reaction of damp heat UC.