The Effects Of PM_2.5 Emitted From Motor Vehicles On The Cellular Toxicity And Global DNA Methylation In EA.hy926 Cell Line

Objective To explore the effects of PM_2.5.5 emitted from motor vehicles on the proliferation function,apoptosis,DNA damage and DNA methylation in EA.hy926 cell line.Methods Motor vehicles PM_2.5.5 was sampled using a fixed-source dilution channel and flow sampler.The PM_2.5.5 suspension was extracted using an ultrasonic water-bath method and then human umbilical vein endothelial cell line（EA.hy926）were treated with various concentrations of it.Cell proliferation,apoptosis,DNA damage,and DNA methylation levels were measured for the cellular toxicity of PM_2.5.Results The proliferation of EA.hy926 cell line is suppressed in 200μg/mL group and there was a statistically significant different（P<0.05）between control group and 200μg/mL group.The cell apoptosis rate and 8-OHdG%of EA.hy926 cell line was found increased and the5-mC%was found decreased.Conclusions PM_2.5.5 emitted from motor vehicles can suppress viability,and cause 8-OHdG%increase and DNA methylation decrease in EA.hy926 cell line.There may no correlation between the variables.