| Objective:Acute lung injury(ALI)is still a big challenge for the clinic,because of the rapid onset,damage to the body,even life-threatening,but its mechanism is not clear,and we lack effective treatment.So the study of acute lung injury has great clinical significance.The datas show that acute lung injury is an acute inflammatory reaction,and therefore,a variety of inflammatory factors has abnormal expression in patients with acute lung injury.Studies have shown that bone mesenchymal stem cells(BMSCs)has great potential value in the treatment of lung injury.Therefore,the basic research and clinical application of acute lung injury has gradually become a hot topic at home and abroad.Therefore,this study used juvenile rat atomization inhalationof lipopolysaccharide(LPS)animal model of acute lung injury was prepared,and the bone marrow mesenchymal stem cells on the treatment of bone marrow mesenchymal stem cells the effects of young ratsl inflammatory factor IL-1 beta,IL-10 on acute lung injury,and MSCs treatment in clinical application provide laboratory evidence.Methods:In this study,we choose male SD SPF young rats(72 rats)as the research object,Rats were randomly divided into 3 groups according to the random number table:control group,disease model group(model group),disease model+MSCs(treatment group),24 rats in each group;at the same time,also it will be randomly divided into 6h12h,24h,48h,a total of 4 sub groups,6 rats in each group.Model group and treatment group(5mg/ml,LPS inhalation dose was 2ml,total continuous inhalation of 20min)the establishment of juvenile rat model of ALI,the control group inhaled normal saline.After1h treatment group by intravenous injection of BMSCs(1 x 10~7/kg,1 x 10~7/ml),model group,control group by intravenous injection of an equal volume of culture medium(DMEM).We observe the pathological changes of lung tissue HE staining,the ratio of W/D and bronchoalveolar lavage fluid(BALF)neutrophil count and protein content,and by changing the content of detection of IL-1 beta and IL-10 in lung tissue by immunohistochemistry and western blot method,through a variety of ways to inhibit inflammatory reaction and reducing effect the mechanism of lung tissue injury of MSCs.Results:In this study,compared with the control group and the treatment group,the amount of neutrophils,the total protein content of the lung tissue,W/D ratio increased foreach time in the model group,the difference is the Statistically significant(P<0.05).After giving the MSCs intervention,the number of neutrophils and total protein The content of the lung injury group was significantly lower than that of the corresponding time point,from the beginning of the 12h node,with statistical significance(P<0.05).Histopathological examination showed that the lung tissue of model group showed typical pathological inflammation changes,including alveolar congestion,hemorrhage,edema,alveolar neutrophil infiltration and vascular wall lesions,alveolar wall thickening degree of lung injury;lung tissue injury treatment group significantly reduced.The contents of IL-1 and IL-10 in model group,control group and treatment group decreased at different time periods.There was significant difference between 48h period and 6h period(P<0.05).After immunohistochemical staining,the expression of IL-1 beta protein in 6h,12h,24h and 48h of lung tissue was significantly expressed in the cytoplasm and cell wall of alveolar epithelial cells in the model group,while the expression intensity of the treatment group decreased significantly.The resuilts of western blot method:Compared with the control group and the treatment group,the content of IL-1 beta and IL-10 protein in the lung tissue of the model group was significantly higher than that of the control group and the treatment group.The difference was statistically significant(P<0.05).The expression of IL-1 beta and IL-10 in the model group and the treatment group showed a tendency to increase first and then decrease,of which IL-1beta was the most significant in 12h,and the most significant of IL-10 in 24h.The expression of IL-10 decreased significantly after 48h,and the difference was statistically significant in the treatment group(P<0.05).Conclusions:Bone marrowmesenchymalstem cellscan significantly affect inflammatory cytokines IL-1 beta and IL-10 in young rats with acute lung injury.The pathogenesis of ALI induced by LPS may be related to the increased expression of IL-1beta and IL-10.After the intervention of BMSCs,the lung tissue damage of ALI young mice was alleviated,and the expression of IL-1 beta and IL-10 decreased significantly compared with the model group,suggesting that BMSCs can alleviate the inflammatory reaction of ALI lung tissue and participate in the repair of lung tissue injury.This argument can be used as an important basis for the clinical treatment of children’s ALI/ARDS,and it is of great significance for the research of later treatment. |
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