Study On The Hypoglycemic Activity Of Water-insoluble Extracts Form Cyclocarya Paliurus (Batal.) Iljin-skaja

Cyclocarya paliurus（Batal.）iljin-skaja is a deciduous tree of the walnut family.It is a special rare and medicinal plant in China.The Modern pharmacological studies have shown that it has a variety of effects such as blood sugar lowering,blood pressure lowering and anti-oxidation.It is prominent in the prevention and treatment of diabetes,and it is a good medicine and food homologous health food.In this paper,the in vitro hypoglycemic activity of water-insoluble extracts（petrol ether,ethyl acetate and n-butanol extracts）of Cyclocarya paliurus was studied usingα-amylase,α-glucosidase and DPPH free radical model;Technical and spectral techniques for the study of the chemical constituents of n-butanol and ethyl acetate extracts from Cyclocarya paliurus;tracking and evaluation of hypoglycemic and antioxidant activities of monomeric compounds in vitro.The results of the study were as follows:1.Different sources（Xiushui Jiangxi,Badagongshan Hunan,Zhangjiajie Hunan,Huangsangpingxiang Suining Hunan,Shaoyang Hunan,Pingyangxiang Rongjiang Guizhou,Xiaodanjiang Rongjiang Guizhou and Guiyang Guizhou）Cyclocarya paliurus petroleum ether extracts for three in vitro models All have inhibitory activity.Among them,the best inhibitory activity ofα-amylase was Badagongshan Hunan（30mg/mL,71.0%）,the best inhibitory activity ofα-glucosidase was Huangsangpingxiang Suining Hunan(IC₅₀为0.037mg/mL),The DPPH free radical scavenging capacity was preferably Xiushui Jiangxi(IC₅₀为0.217 mg/mL).2.The change of the content of anthraquinones in the petroleum ether fraction of Cyclocarya paliurus showed a trend of“high-low-high-high”,among which the content of total terpenes was the highest in September,while the inhibitory activity was the best in April,and the overall inhibition rate was over 40%.3.Using a variety of chromatographic separation techniques and spectroscopic techniques,10 monomer compounds were isolated and prepared.They are Panaxerol B,Panaxerol C,Cyclocarioside H,Cyclocarioside F,Quercetin,Chlorogenicacidbutylester,quercetin-3-O-β-D-glucuronic acid-6"-n-butyl ester,Kaempferol 3-（6-butylglucuronide）,K-aempferol 3-（6-methyl glucuronide）,neochlorogenic acid butyl ester.4.In vitro hypoglycemic and antioxidant model tests were used as evaluation indicators to determine 9 individual compounds.The results showed that n-butanol,extract,quercetin,Kaempferol3-（6-Butylglucuronid-e）andChlorogenicacidbutylester,quercetin-3-O-β-D-glucuronic acid-6"-n-butyl ester has a strong inhibitory effect onα-glucosidase activity,IC₅₀0 size of quercetin（0.093mg/mL）>quercetin-3-O-β-D-glucuro-nic acid-6"-n-butyl ester（0.357mg/mL）>Kaempferol 3-（6-butylglucuro-nide）（0.388 mg/mL）>n-butanol extract（0.486 mg/mL）,and other compounds inhibited activity is relatively weak;DPPH free radical scavenging results showed that the clearance rate of the highest concentration of 400μg/mL was respectively,butyl chlorogenic acid（57.9%）,uercetin-3-O-β-D-glucuronic acid-6"-n-butyl ester（74.4%）,ethyl acetate extractt（72.3%）and n-butanol extract（88%）of which quercetin have strong scavenging ability.The IC₅₀ value is quercetin（2.32μg/mL）>VC（4.22μg/mL）.The scavenging rate of other compounds is relatively weak.