| [Objective]to investigate the effect of methamphetamine on spermatogenesis and its possible mechanism in pubertal male rats.By observing the general condition of rats,the weight growth of rats was monitored,and the testicular coefficient,epididymal coefficient,sperm quantity,sperm motility rate,sperm abnormality rate and serum inhibin B level were detected,and the changes of testicular histology were observed.To provide scientific data and theoretical support for elucidating the effect of methamphetamine abuse on male spermatogenesis and related mechanisms.[Methods]A total of 40 male SD rats(Sprague Dawley,SD),50 days old(puberty),were selected for adaptive feeding for one week.They were randomly divided into four groups:A,B,C and D.Group A:8 weeks exposure group;B group:8 week control group;C group:12 weeks poison group;D group:12 week control group.There were 10 rats in each group,group A and group C were injected intraperitoneally with methamphetamine normal saline according to the dosage of 10mg/kg,and group B and group D were given intraperitoneal injection of 0.9%normal saline.Give the medicine once a day.After being weighed once a week,the rats were weighed once in each group.After the rats were poisoned,the rats were stopped for 24h,and then fasting(can not help water)for 12 hours,weighing the body weight,collecting the abdominal aorta blood,dislocating the cervical vertebra,weighing the bilateral testicles and epididymis weight,calculating the testis coefficient and epididymal number.And four groups of A,B,C and D were used to make HE staining sections of testicular tissue in rats.The morphological and structural changes of testicular germ cells were observed under light microscope.One side epididymis was selected to prepare epididymal sperm suspension,and the number of sperm in each gram epididymal tissue was calculated.Sperm smear was used to observe sperm morphology and calculate the rate of malformation.Count the number of motile sperm in 200 sperm and calculate sperm motility.The content of inhibin B(Inhibin B,INHB)in serum samples was detected.[results](1)the weight of rats:first weeks,2 weeks,4 weeks,8 weeks and 12 weeks,the body weight of group C rats was compared with that of group D,the weight of the two rats increased,but the weight of the group C rats was faster than the D group,and the difference was statistically significant(p<0.05).(2)the coefficient of testicle and epididymis:group A and group B,and the coefficient of testis and epididymis in group C and D group decreased,and the difference was statistically significant(p<0.05).(3)sperm quantity:the number of spermatozoa in each gram epididymal tissue of group A rats was not significantly changed,the difference was not statistically significant(p>0.05).The number of sperm in each gram epididymal tissue of group C rats was significantly lower than that in the D group,the difference was statistically significant(p<0.05).(4)sperm motility rate:the rate of sperm activity in the A group was lower than that in the B group,but the difference was not statistically significant(p>0.05);the sperm activity rate in the C group was significantly lower than that in the D group,and the difference was statistically significant(p<0.05).(5)sperm abnormality rate:the rate of sperm malformation in group A was slightly higher than that in group B,but the difference was not statistically significant(p>0.05);the rate of sperm malformation in group C was significantly higher than that in D group,and the difference was statistically significant(p<0.05).(6)HE staining results of testis tissue:the epithelial layer of testicular spermatogenic tube in A and C two rats was obviously decreased,only 2-3 layers,the cells were loosely arranged,some of the permutations were disordered,interstitial edema,some spermatogonial cells fall off,the spermatocytes can be seen vacuolization and edema in the spermatocytes,and some spermatocytes appear necrotic.(7)the level of serum inhibin B in rats:the A group was slightly lower than the B group,but the difference was not statistically significant(p>0.05).The content of serum inhibin B in the C group was significantly lower than that in the D group,and the difference was statistically significant(P<0.05).[Conclusion(s)]1.a rat model of infection can be established by intraperitoneal injection of methamphetamine.2.methamphetamine can cause pathological damage of germ cells in pubertal SD male rats.Methamphetamine can cause the decrease of testis and epididymis coefficient,epididymal sperm count,serum inhibin B level and sperm motility rate in pubertal SD male rats.3.methamphetamine can increase the rate of sperm malformation in adolescent SD male rats.The above conclusion shows that the function of spermatogenesis in rats is decreased by intraperitoneal injection of methamphetamine,and its mechanism may reduce the secretion of serum inhibin B by injuring the testis supporting cells,the seminiferous tubule complex,and reducing the Spermatogenesis Function of rats. |
PDF Full Text Request