Expression Characteristics Of Peripheral Blood PD-1 And PD1.5 Polymorphism In Han And Kazak Atrial Fibrillation Patients Of Xinjiang

Objective:Atrial fibrillation(AF)is one of the most common arrhythmia.In recent years,the role of PD-1 in occurrence and development of atrial fibrillation(AF)has arouse highly attention.This study aimed to examine the peripheral blood PD-1expression,and investigate the correlation between the PD1.5 polymorphisms in PD-1gene and atrial fibrillation(AF)occurrence in Han and Kazakh populations of Xinjiang,to further clarify the role of PD-1 in atrial fibrillation development.Methods:100 Han and 100 Kazakh patients with atrial fibrillation were selected as case group respectively,and 100 Han and 100 Kazakh non-AF patients were selected as control group simultaneously.Peripheral blood samples were collected to detect the PD-1 expression on the surface of CD4~+T cell and extract DNA.Then,genotype and allele frequency of PD-1gene PD1.5 gene loci were determined using polymerase reaction(PCR)and electrophoresis technology.Results:Both in Han and Kazakh population,PD-1expression on peripheral blood CD4+T cells has a statistically significant difference between the atrial fibrillation group and control group(P<0.00).PD 1.5 gene locus includes CC wild-type,CT heterozygote,TT complete mutant type.In Han and Kazakh population,genotype and allele frequencies had no statistical difference between atrial fibrillation group and non-atrial fibrillation group(P>0.05).Gene frequency and genotype comparison results showed significant statistically difference in genotype between Han and Kazakh population(P<0.05).Logistic regression analysis showed that the PD-1 expression rate on CD4+T cells may be an independent protective factor for atrial fibrillation,in total population(OR=0.73,95%CI=0.63-0.85,P=0.000),in Kazakh(OR=0.66,95%CI=0.50-0.87,P=0.003),and in Han(OR=0.72,95%CI=0.59-0.87,P=0.001).Conclusion:The results suggest the correlation between PD-1expression on peripheral blood CD4+T cells and atrial fibrillation prevalence in the Han and Kazakh population in China.PD-1 gene PD1.5 polymorphism has no relevance with the atrial fibrillation.