Based On The Nrf2/ARE Signaling Pathway To Explore The Effect Of Kelong Capsule On Benign Prostatic Hyperplasia Rats

Objective: To evaluate the effects of Kelong Capsule(KLC)on testosterone-induced benign prostatic hyperplasia(BPH)and Nrf2/ARE signaling pathway in rat model.The prostate index,sex hormone level,the protein and m RNA expression of Nrf2 and its downstream antioxidant were compared in different groups.To explore the potential mechanism of KLC in the treatment of BPH,and provide theoretical basis and clinical guidance for the prevention and treatment of BPH.Method: Forty male SD rats were randomly divided into a control group,model group,low-dose KLC(3.6 g/kg),high-dose KLC(7.2 g/kg),t BHQ group.The rats were injected with testosterone propionate(3 mg/kg)and drug intervention for 28 days.The prostate index was calculated.ELISA kit was used to detect the content of testosterone(T),estradiol(E2)and glutathione(GSH).Western blot and RT-PCR were used to detect the expression level of Nrf2,HO-1 and NQO1 in rat prostate tissue.Result:Compared with the blank group,the wet weight,PI and T contents in the model group were increased significantly,the GSH content was decreased(P<0.05 or P<0.01).Pathological examination showed that the epithelial layer in the model group.The protein expression of Nrf2,HO-1 and NQO1 of prostate tissue in the model group was decreased obviously(P<0.01),the m RNA expression of Nrf2 and NQO1 was down regulated(P<0.05 or P<0.01).Compared with the model group,the prostate wet weight,PI,T and E2 of high-dose KLC group was decreased,and the content of GSH was increased(P<0.05 or P<0.01);inhibited prostate epithelial hyperplasia.Low-dose KLC group up-regulated the protein expression of Nrf2 and HO-1(P<0.01),and significantly induced the m RNA expression of Nrf2 NQO1,HO-1(P<0.05 or P<0.01).High-dose KLC down-regulated the protein expression of HO-1 and NQO1 and NQO1 m RNA expression(P<0.05 or P<0.01).Conclusion: The BPH rat model was successfully established by testosterone injection.KLC reduced PI,improved the pathological structure of prostate tissue.KLC significantly inhibited the hyperplasia of prostate in rats.Low-dose KLC could increase the activities of antioxidant enzymes by activating Nrf2/ARE signaling pathway.